中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2009年
4期
257-259
,共3页
流产习惯性%T淋巴细胞调节性%树突细胞%细胞因子
流產習慣性%T淋巴細胞調節性%樹突細胞%細胞因子
유산습관성%T림파세포조절성%수돌세포%세포인자
Abortion,habitual%T-limphocytes,regulatory%Dendritic cells%Cytokines
目的 探讨原因不明复发性流产(URSA)患者外周血和蜕膜组织中CD+4CD+25调节性T淋巴细胞(Tr细胞)对树突状细胞(DC)的调控作用.方法 采集4例URSA患者(流产组)和4例正常早孕妇女(对照组)的外周血和蜕膜组织,密度梯度离心法、免疫磁珠分离法分选出Tr细胞和DC,培养6 d,培养方法分为DC单独培养和Tr细胞与Dc混合培养.酶联免疫吸附试验(ELISA)检测两种方法培养的细胞培养上清液中辅助性T淋巴细胞(Th)1型细胞因子--γ干扰素(IFN-γ)的Th2型细胞因子--白细胞介素10(IL-10)的蛋白含量.结果 (1)外周血:流产组细胞混合培养和单独培养后,细胞培养上清液中IFN-γ蛋白含量为(22.5±3.0)、(23.2±0.7)ng/L,两者比较,差异无统计学意义(P>0.05);IL-10蛋白含量分别为(35±4)、(37±7)ng/L,两者比较,差异也无统计学意义(P>0.05).对照组细胞混合培养和单独培养后,IFN-γ蛋白含量为(36±1.1)、(30.5±4.0)ns/L,两者比较,差异无统计学意义(P>0.05);IL-10蛋白含量分别为(36±9)、(54±20)ng/L,两者比较,差异有统计学意义(P<0.01).(2)蜕膜组织:流产组细胞混合培养和单独培养后,IFN-γ蛋白含量分别为(24.4±2.5)、(23.4±2.6)ng/L,两者比较,差异无统计学意义(P>0.05);IL-10蛋白含量分别为(25±5)、(28±7)ng/L,两者比较,差异也无统计学意义(P>0.05);对照组细胞混合培养后,IFN-γ蛋白含量为(22.6±3.8)ng/L,明显低于单独培养后的(30.7±4.6)ng/L,差异有统计学意义(P<0.01);对照组细胞混合培养后,IL-10蛋白含量为(31±9)ng/L,明显高于单独培养后的(27±6)ng/L,差异也有统计学意义(P<0.05).结论 URSA患者Tr细胞抑制性免疫功能下降,从而导致对DC调控失常,Th1/Th2平衡失调和母胎免疫耐受异常.
目的 探討原因不明複髮性流產(URSA)患者外週血和蛻膜組織中CD+4CD+25調節性T淋巴細胞(Tr細胞)對樹突狀細胞(DC)的調控作用.方法 採集4例URSA患者(流產組)和4例正常早孕婦女(對照組)的外週血和蛻膜組織,密度梯度離心法、免疫磁珠分離法分選齣Tr細胞和DC,培養6 d,培養方法分為DC單獨培養和Tr細胞與Dc混閤培養.酶聯免疫吸附試驗(ELISA)檢測兩種方法培養的細胞培養上清液中輔助性T淋巴細胞(Th)1型細胞因子--γ榦擾素(IFN-γ)的Th2型細胞因子--白細胞介素10(IL-10)的蛋白含量.結果 (1)外週血:流產組細胞混閤培養和單獨培養後,細胞培養上清液中IFN-γ蛋白含量為(22.5±3.0)、(23.2±0.7)ng/L,兩者比較,差異無統計學意義(P>0.05);IL-10蛋白含量分彆為(35±4)、(37±7)ng/L,兩者比較,差異也無統計學意義(P>0.05).對照組細胞混閤培養和單獨培養後,IFN-γ蛋白含量為(36±1.1)、(30.5±4.0)ns/L,兩者比較,差異無統計學意義(P>0.05);IL-10蛋白含量分彆為(36±9)、(54±20)ng/L,兩者比較,差異有統計學意義(P<0.01).(2)蛻膜組織:流產組細胞混閤培養和單獨培養後,IFN-γ蛋白含量分彆為(24.4±2.5)、(23.4±2.6)ng/L,兩者比較,差異無統計學意義(P>0.05);IL-10蛋白含量分彆為(25±5)、(28±7)ng/L,兩者比較,差異也無統計學意義(P>0.05);對照組細胞混閤培養後,IFN-γ蛋白含量為(22.6±3.8)ng/L,明顯低于單獨培養後的(30.7±4.6)ng/L,差異有統計學意義(P<0.01);對照組細胞混閤培養後,IL-10蛋白含量為(31±9)ng/L,明顯高于單獨培養後的(27±6)ng/L,差異也有統計學意義(P<0.05).結論 URSA患者Tr細胞抑製性免疫功能下降,從而導緻對DC調控失常,Th1/Th2平衡失調和母胎免疫耐受異常.
목적 탐토원인불명복발성유산(URSA)환자외주혈화세막조직중CD+4CD+25조절성T림파세포(Tr세포)대수돌상세포(DC)적조공작용.방법 채집4례URSA환자(유산조)화4례정상조잉부녀(대조조)적외주혈화세막조직,밀도제도리심법、면역자주분리법분선출Tr세포화DC,배양6 d,배양방법분위DC단독배양화Tr세포여Dc혼합배양.매련면역흡부시험(ELISA)검측량충방법배양적세포배양상청액중보조성T림파세포(Th)1형세포인자--γ간우소(IFN-γ)적Th2형세포인자--백세포개소10(IL-10)적단백함량.결과 (1)외주혈:유산조세포혼합배양화단독배양후,세포배양상청액중IFN-γ단백함량위(22.5±3.0)、(23.2±0.7)ng/L,량자비교,차이무통계학의의(P>0.05);IL-10단백함량분별위(35±4)、(37±7)ng/L,량자비교,차이야무통계학의의(P>0.05).대조조세포혼합배양화단독배양후,IFN-γ단백함량위(36±1.1)、(30.5±4.0)ns/L,량자비교,차이무통계학의의(P>0.05);IL-10단백함량분별위(36±9)、(54±20)ng/L,량자비교,차이유통계학의의(P<0.01).(2)세막조직:유산조세포혼합배양화단독배양후,IFN-γ단백함량분별위(24.4±2.5)、(23.4±2.6)ng/L,량자비교,차이무통계학의의(P>0.05);IL-10단백함량분별위(25±5)、(28±7)ng/L,량자비교,차이야무통계학의의(P>0.05);대조조세포혼합배양후,IFN-γ단백함량위(22.6±3.8)ng/L,명현저우단독배양후적(30.7±4.6)ng/L,차이유통계학의의(P<0.01);대조조세포혼합배양후,IL-10단백함량위(31±9)ng/L,명현고우단독배양후적(27±6)ng/L,차이야유통계학의의(P<0.05).결론 URSA환자Tr세포억제성면역공능하강,종이도치대DC조공실상,Th1/Th2평형실조화모태면역내수이상.
Objective To studythe effect of CD+4CD+25 regulatory T(Tr)cells on dendric cells(DC)in peripheral blood and deciduas from unexplained recurrent spontaneous abortion(URSA)patients.Methods Four URSA patients(abortion group)and 4 normal early pregnant women(control group)were enrolled in this study.Tr cells and DC in the peripheral blood and deciduas were isolated using Ficoll density gradient centrifugation and magnetic cell sorting(MACS).DC were cultured alone(DC alone)or in combination with Tr cells(DC+Tr)for 6 days,during which the release of interferon(IFN)-γandinterleukin(IL)-10 in the medium was subsequently measured by enzyme linked immunoadsorbent assay (ELISA).Results(1) Peripheral blood:there was no significant difference in IFN-γlevel between DCalone(23.2±0.7)ng/L and DC+Tr(22.5±3.0)ng/L in abortion group(P>0.05).The similar level of IL-10 was observed between DC alone(37±7)ng/L and DC+Tr(35±4)ng/L in abortion group(P>0.05).IL-10 level,but not IFN-γ,was significantly hisber in DC alone(54±20)ns/L than that in DC+ Tr(36±9)ng/L in control group(P<0.01).(2)Deciduas:there was no significant difference in IFN-γlevel between DC alone(23.4±2.6)ng/L and DC+Tr(24.4±2.5)ng/L in abortion group(P>0.05).Moreover,Similar IL-10 level was found between DC alone(28±7)ng/L and DC+Tr(25±5)ng/L in abortion group(P>0.05).IFN-γlevel in CD alone(30.7±4.6)ng/L was significantly higher than that in DC+Tr(22.6±3.8)ng/L in control group(P<0.01);whereas IL-10 level was much lower in DC alone (27±6)ng/L than that in DC+Tr(31±9)ng/L in control group(P<0.05).Conclusion The decreasing of immunosuppressive funetion of Tr eell of URSA patients affect its regulation on DC.resulting in imbalance of Th1/Th2 and abnormality of maternal-fetal jmmuno-tolerence in URSA.
