中华外科杂志
中華外科雜誌
중화외과잡지
CHINESE JOURNAL OF SURGERY
2008年
16期
1249-1252
,共4页
崔建德%梁双武%裴国献%江汕%戴金良%刘勇%姚旺祥%黄爱文%王秋实%赵培冉
崔建德%樑雙武%裴國獻%江汕%戴金良%劉勇%姚旺祥%黃愛文%王鞦實%趙培冉
최건덕%량쌍무%배국헌%강산%대금량%류용%요왕상%황애문%왕추실%조배염
组织工程%骨%血管束%感觉神经束%降钙素基因相关肽%神经肽Y
組織工程%骨%血管束%感覺神經束%降鈣素基因相關肽%神經肽Y
조직공정%골%혈관속%감각신경속%강개소기인상관태%신경태Y
Tissue engineering%Bones%Vascular bundle%Sensory nerve tract%Calcitonin gene related peptide%Neuropeptide Y
目的 比较单纯血管、神经束分别植入组织工程骨修复兔骨缺损对神经肽降钙素基因相关肽(calcitonin gene related peptide,CGRP)和神经肽Y(neuropeptide Y,NPY)表达影响. 方法 36只新西兰大白兔,随机平均分为3组:A组为组织工程骨+股血管束植入,B组为组织工程骨+感觉神经束植入,C组为单纯组织工程骨.每只动物均在右侧股骨制作长1.5 cm的段缺性骨与骨膜缺损,钢板固定后分别用以上方法修复大段骨缺损.术后3、6、12个月行免疫组化染色检测修复骨段内的CGRP和NPY的表达,并采用图像分析软件行半定量分析. 结果 CGRP和NPY的表达量在A、B、C 3组均随时间推移而增多(P=0.000),在3个月时A组较B组多(P=0.000),但在6个月和12个月时A组和B组的表达量无明显差异(P>0.05).A、B两组在3、6、12个月时的表达最均较C组多(P=0.000). 结论 与单纯神经束植入组相比,单纯血管束植入组织工程骨修复兔骨缺损在术后3个月可以明显促进CGRP和NPY表达,但后期两组对神经肽表达的影响作用无明显差异.
目的 比較單純血管、神經束分彆植入組織工程骨脩複兔骨缺損對神經肽降鈣素基因相關肽(calcitonin gene related peptide,CGRP)和神經肽Y(neuropeptide Y,NPY)錶達影響. 方法 36隻新西蘭大白兔,隨機平均分為3組:A組為組織工程骨+股血管束植入,B組為組織工程骨+感覺神經束植入,C組為單純組織工程骨.每隻動物均在右側股骨製作長1.5 cm的段缺性骨與骨膜缺損,鋼闆固定後分彆用以上方法脩複大段骨缺損.術後3、6、12箇月行免疫組化染色檢測脩複骨段內的CGRP和NPY的錶達,併採用圖像分析軟件行半定量分析. 結果 CGRP和NPY的錶達量在A、B、C 3組均隨時間推移而增多(P=0.000),在3箇月時A組較B組多(P=0.000),但在6箇月和12箇月時A組和B組的錶達量無明顯差異(P>0.05).A、B兩組在3、6、12箇月時的錶達最均較C組多(P=0.000). 結論 與單純神經束植入組相比,單純血管束植入組織工程骨脩複兔骨缺損在術後3箇月可以明顯促進CGRP和NPY錶達,但後期兩組對神經肽錶達的影響作用無明顯差異.
목적 비교단순혈관、신경속분별식입조직공정골수복토골결손대신경태강개소기인상관태(calcitonin gene related peptide,CGRP)화신경태Y(neuropeptide Y,NPY)표체영향. 방법 36지신서란대백토,수궤평균분위3조:A조위조직공정골+고혈관속식입,B조위조직공정골+감각신경속식입,C조위단순조직공정골.매지동물균재우측고골제작장1.5 cm적단결성골여골막결손,강판고정후분별용이상방법수복대단골결손.술후3、6、12개월행면역조화염색검측수복골단내적CGRP화NPY적표체,병채용도상분석연건행반정량분석. 결과 CGRP화NPY적표체량재A、B、C 3조균수시간추이이증다(P=0.000),재3개월시A조교B조다(P=0.000),단재6개월화12개월시A조화B조적표체량무명현차이(P>0.05).A、B량조재3、6、12개월시적표체최균교C조다(P=0.000). 결론 여단순신경속식입조상비,단순혈관속식입조직공정골수복토골결손재술후3개월가이명현촉진CGRP화NPY표체,단후기량조대신경태표체적영향작용무명현차이.
Objective To evaluate the different effect on the expression of Calcitonin gene related peptide (CGRP)and neuropeptide Y (NPY) between tissue engineered bone with vascular bundle graft in vivo and that with sensory nerve tract graft in vivo. Method Thirty-six healthy New Zealand rabbits were divided into 3 goups randomly and equally: vascular bundle group ( A), sensory nerve tract group ( B), tissue-engineering group (C). Group A segmental bone defect of 1.5 cm long was made at the right femur in each animal. After plate fixation, the defects were implanted respectively with the engineered bone prepared in the above-mentioned 3 methods. At 3, 6 and 12 months pest-operatively, the distribution of CGRP and NPY in the new bone were detected by immunohistochemistry and analyzed semi-quantatively by image analysis software. Results CGRP and NPY immuno-histochemical results indicated their contents increased significantly in all 3 groups as time passed( P =0. 000). Compared with group B, the contents of CGRP and NPY in group A significantly increased at 3 months ( P = 0.000), but there was no statistic difference between them at 6 or 12 months( P >0. 05 ). The expression of CGRP and NPY in both group A and B were significantly more than that in group C at 3,6 or 12 months (P =0. 000). Conclusion Implantation of vascular bundle into tissue-engineered bone can significantly improve the CGRP and NPY contents at early 3 months comparing with Implantation of sensory tract into tissue-engineered bone, but the changes are not significant at 6 or 12 months post-operatively.
