CAJ | 학술논문

目的观察大豆异黄酮在不同的雌激素环境下,对MMTV-erbB-2转基因小鼠的乳腺肿瘤发生及发展的影响.方法选取鼠龄5周的健康MMTV-erbB-2转基因雌性小鼠150只,分为对照组、低雌激素组1、低雌激素组2、高雌激素组1和高雌激素组2五组,观察各组小鼠乳腺癌的发病率和潜伏期,并采用免疫组织化学染色SP法检测各组小鼠乳腺组织中雌激素受体(ER)、孕激素受体(PR)、增殖细胞核抗原(PCNA)的表达.结果对照组、低雌激素组1、低雌激素组2、高雌激素组1和高雌激素组2的乳腺癌发病率分别为73.3%、96.7%、30.3%、40.0%和83.3%,对照组与高雌激素组2、低雌激素组1与高雌激素组2发病率比较差异无统计学意义(P＞0.05),其他各组间比较差异有统计学意义(P＜0.05).各组小鼠乳腺癌平均潜伏期比较差异无统计学意义(P＞0.05).低雌激素组1的TEB数量与其他各组比较差异有统计学意义(P＜0.05).erbB-2表达在各组之间表达差异无统计学意义(P＞0.05).低雌激素组1、低雌激素组2、高雌激素组1和高雌激素组2与对照组乳腺ER和PR的表达比较差异无统计学意义(P＞0.05).低雌激素组1实验小鼠发生肿瘤的乳腺组织内PCNA的表达明显高于其他各组(P＜0.05).对照组与高雌激素组2,低雌激素组1与高雌激素组2比较差异有统计学意义(P＜0.05),其他各组间比较差异有统计学意义(P＜0.05).结论大豆异黄酮在不同的雌激素环境下,对MMTV-erbB-2转基因小鼠乳腺肿瘤的发生及发展起不同的作用.低雌激素环境下大豆异黄酮可促进乳腺肿瘤的发生及发展,高雌激素环境下大豆异黄酮可抑制乳腺肿瘤的发生及发展.
목적 관찰대두이황동재불동적자격소배경하,대MMTV-erbB-2전기인소서적유선종류발생급발전적영향.방법 선취서령5주적건강MMTV-erbB-2전기인자성소서150지,분위대조조、저자격소조1、저자격소조2、고자격소조1화고자격소조2오조,관찰각조소서유선암적발병솔화잠복기,병채용면역조직화학염색SP법검측각조소서유선조직중자격소수체(ER)、잉격소수체(PR)、증식세포핵항원(PCNA)적표체.결과 대조조、저자격소조1、저자격소조2、고자격소조1화고자격소조2적유선암발병솔분별위73.3%、96.7%、30.3%、40.0%화83.3%,대조조여고자격소조2、저자격소조1여고자격소조2발병솔비교차이무통계학의의(P＞0.05),기타각조간비교차이유통계학의의(P＜0.05).각조소서유선암평균잠복기비교차이무통계학의의(P＞0.05).저자격소조1적TEB수량여기타각조비교차이유통계학의의(P＜0.05).erbB-2표체재각조지간표체차이무통계학의의(P＞0.05).저자격소조1、저자격소조2、고자격소조1화고자격소조2여대조조유선ER화PR적표체비교차이무통계학의의(P＞0.05).저자격소조1실험소서발생종류적유선조직내PCNA적표체명현고우기타각조(P＜0.05).대조조여고자격소조2,저자격소조1여고자격소조2비교차이유통계학의의(P＜0.05),기타각조간비교차이유통계학의의(P＜0.05).결론 대두이황동재불동적자격소배경하,대MMTV-erbB-2전기인소서유선종류적발생급발전기불동적작용.저자격소배경하대두이황동가촉진유선종류적발생급발전,고자격소배경하대두이황동가억제유선종류적발생급발전.
Objective To investigate the effect of the soybean isoflavones at different estrogen environments on the pathogenesis of breast cancer in MMTV-erbB-2 transgenic mice. Methods 150 fiveweek-old MMTV-erbB-2 transgenic female mice were phosen and divided into five groups; control group,low estrogen group 1, low estrogen group 2, high estrogen group 1 and high estrogen group 2. The incidence and latent period of breast cancer were observed, and the expression of estrogen receptor (ER) ,progesterone receptor (PR), and proliferating cell nuclear antigen (PCNA) proteins was detected by using immunohistochemistry SP method. Results The incidence of breast cancer in control group, low estrogen group 1, low estrogen group 2, high estrogen group 1 and high estrogen group 2 was 73. 3% , 96. 7% ,30. 3% , 40. 0% and 83. 3% , respectively, with the difference being not significant between control group and high estrogen group 2, and between low estrogen group 1 and high estrogen group 2 (P ＞ 0. 05 ) , but with the difference being significant among the other groups (P ＜ 0. 05 ). There was significant difference in the average latent period of breast cancer among all groups (P ＞ 0. 05 ). There was significant difference in the number of TEB between low-estrogen group 1 and other groups (P ＜ 0.05). There was significant difference in the erbB-2 expression among the groups ( P ＞ 0. 05 ). There was no significant difference in the expression of breast ER and PR between control group and other groups ( P ＞ 0. 05 ). The PCNA expression in breast tumor tissue in low-estrogen group 1 was significantly higher than other groups (P ＜0. 05) , and there was significant difference in the PCNA expression between control group and high estrogen group 2, between low estrogen group 1 and high estrogen group 2 (P ＜ 0. 05). Conclusion The soybean isoflavones at different estrogen environments play different roles in the occurrence and development of MMTV-erbb-2 transgenic mouse mammary tumor. In the context of low estrogen, soybean isoflavones could even promote breast cancer formation and development. In the context of high estrogen, soybean isoflavones could inhibit breast cancer and development.