CAJ | 학술논문

目的研究TRAIL受体在肝癌组织及肝癌细胞中的表达,探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)及其受体在肝癌细胞凋亡诱导中的作用.方法应用RT-PCR法和免疫组化方法检测肝癌组织及细胞系SK-Hep1中TRAIL受体的表达,并以TRAIL蛋白和γ-干扰素作用于SK-Hep1细胞,观察其对细胞的凋亡诱导作用.结果在肝癌组织及肝癌SK-Hep1细胞系中均可检测到TRAIL受体,其中,死亡受体DR4、DR5在肝癌、癌旁组织以及SK-Hep1肝癌细胞系中均呈高表达,而诱骗受体DcR1及DcR2呈低表达,明显低于正常组织.TRAIL对肝癌细胞有凋亡诱导作用,与干扰素的协同时,其作用明显增强.结论肝癌组织中存在着TRAIL受体的表达,TRAIL可诱导肝癌SK-Hep1细胞系凋亡,其作用与DR4、DR5的高表达有关.
목적연구TRAIL수체재간암조직급간암세포중적표체,탐토종류배사인자상관조망유도배체(TRAIL)급기수체재간암세포조망유도중적작용.방법응용RT-PCR법화면역조화방법검측간암조직급세포계SK-Hep1중TRAIL수체적표체,병이TRAIL단백화γ-간우소작용우SK-Hep1세포,관찰기대세포적조망유도작용.결과재간암조직급간암SK-Hep1세포계중균가검측도TRAIL수체,기중,사망수체DR4、DR5재간암、암방조직이급SK-Hep1간암세포계중균정고표체,이유편수체DcR1급DcR2정저표체,명현저우정상조직.TRAIL대간암세포유조망유도작용,여간우소적협동시,기작용명현증강.결론간암조직중존재착TRAIL수체적표체,TRAIL가유도간암SK-Hep1세포계조망,기작용여DR4、DR5적고표체유관.
Objective: To investigate the expression of TRAIL receptors in human hepatocellular carcinomaand neighboring non-tumor liver tissues and to study its roles in apoptosis of SK-Hep1 cell line. Methods:The expression of TRAIL receptors in hepatocellular carcinoma and in SK-Hep1 cell line was detected byimmunohistochemical staining and RT-PCR, and the apoptosis of SK-Hep1 cell line induced by recombi-nant human TRAIL together with or without INF-γ was measured by FCM. Results: The expression offour TRAIL receptors was detectable in human hepatocellular carcinoma and neighboring non-tumor livertissues. High expression of DR4 and DR5 was detected in tumor and neighboring non-tumor liver tissueswhereas low expression of DcR1 and DcR2 was found in tumor, significantly lower than that in normalliver tissues (67.86% vs 100%, 78.57% vs 100%, respectively, P＜0.01. TRAIL together with INF-γ stronglyincreased the apoptosis of SK Hep1 cell line. Conclusion: The four TRAIL receptors can be detected inhepatocellular carcinoma and in SK-Hep1 cell line. Combinations of TRAIL and INF-γ may be a usefulalternative to HCC.