CAJ | 학술논문

目的:从粗糙表面、多孔表面、规则刻槽表面、纤维表面、纹理结构和蛋白质层6个方面全面论述组织工程材料表面拓扑结构对细胞相容性的影响.资料来源:应用计算机检索PUBMED 1987-01/2007-01的相关文章.检索词为 "bio-compatibility, bio-compatibility materials,tissue engineering,tissue engineering materials, cell-compatibility, cell-compatibility materials",并限定文章语言种类为English.资料选择:对资料进行初审,并查看每篇文献后的引文.纳入标准:文章所述内容应与生物相容性组织工程材料相关.排除标准:重复研究或Meta分析类文章.资料提炼:共收集到74篇相关文献,32篇文献符合纳入标准,排除的42篇文献为内容陈旧或重复.符合纳入标准的32篇文献中,24篇涉及生物相容性,8篇涉及细胞相容性材料.资料综合:①关于组织工程材料与生物体的相互作用:综述了高分子组织工程材料在与生物体组织接触时发生各种各样的相互作用.指出了材料与生物体的相互作用情况决定了材料组织相容性的程度;材料对组织相容性的影响包含着微观分子水平和宏观尺度水平,而且,宏观大尺度上(包括材料表面的拓扑结构)的效应比分子尺度上发生的化学效应更为重要.②关于材料表面物理化学性质对材料细胞相容性的影响:综述了高分子材料的粗糙表面、多孔表面、规则刻槽表面、纤维表面、纹理结构和蛋白质层等材料表面的拓扑结构对材料细胞相容性的影响.指出这种影响是研究组织工程材料的生物相容性及进行组织相容性材料设计的重要内容.结论:材料表面的拓扑结构对材料的细胞相容性有着较大影响,细胞与聚合物的相互作用是评价材料细胞相容性的指标.细胞与高分子材料间短期的相互作用程度可由细胞与高分子材料表面的黏附程度来评价,而长期的相互作用可以通过检测体外培养细胞的生长情况或在体内植入高分子村料进行长期评估. 目的:從粗糙錶麵、多孔錶麵、規則刻槽錶麵、纖維錶麵、紋理結構和蛋白質層6箇方麵全麵論述組織工程材料錶麵拓撲結構對細胞相容性的影響.資料來源:應用計算機檢索PUBMED 1987-01/2007-01的相關文章.檢索詞為 "bio-compatibility, bio-compatibility materials,tissue engineering,tissue engineering materials, cell-compatibility, cell-compatibility materials",併限定文章語言種類為English.資料選擇:對資料進行初審,併查看每篇文獻後的引文.納入標準:文章所述內容應與生物相容性組織工程材料相關.排除標準:重複研究或Meta分析類文章.資料提煉:共收集到74篇相關文獻,32篇文獻符閤納入標準,排除的42篇文獻為內容陳舊或重複.符閤納入標準的32篇文獻中,24篇涉及生物相容性,8篇涉及細胞相容性材料.資料綜閤:①關于組織工程材料與生物體的相互作用:綜述瞭高分子組織工程材料在與生物體組織接觸時髮生各種各樣的相互作用.指齣瞭材料與生物體的相互作用情況決定瞭材料組織相容性的程度;材料對組織相容性的影響包含著微觀分子水平和宏觀呎度水平,而且,宏觀大呎度上(包括材料錶麵的拓撲結構)的效應比分子呎度上髮生的化學效應更為重要.②關于材料錶麵物理化學性質對材料細胞相容性的影響:綜述瞭高分子材料的粗糙錶麵、多孔錶麵、規則刻槽錶麵、纖維錶麵、紋理結構和蛋白質層等材料錶麵的拓撲結構對材料細胞相容性的影響.指齣這種影響是研究組織工程材料的生物相容性及進行組織相容性材料設計的重要內容.結論:材料錶麵的拓撲結構對材料的細胞相容性有著較大影響,細胞與聚閤物的相互作用是評價材料細胞相容性的指標.細胞與高分子材料間短期的相互作用程度可由細胞與高分子材料錶麵的黏附程度來評價,而長期的相互作用可以通過檢測體外培養細胞的生長情況或在體內植入高分子村料進行長期評估.
목적:종조조표면、다공표면、규칙각조표면、섬유표면、문리결구화단백질층6개방면전면논술조직공정재료표면탁복결구대세포상용성적영향.자료래원:응용계산궤검색PUBMED 1987-01/2007-01적상관문장.검색사위 "bio-compatibility, bio-compatibility materials,tissue engineering,tissue engineering materials, cell-compatibility, cell-compatibility materials",병한정문장어언충류위English.자료선택:대자료진행초심,병사간매편문헌후적인문.납입표준:문장소술내용응여생물상용성조직공정재료상관.배제표준:중복연구혹Meta분석류문장.자료제련:공수집도74편상관문헌,32편문헌부합납입표준,배제적42편문헌위내용진구혹중복.부합납입표준적32편문헌중,24편섭급생물상용성,8편섭급세포상용성재료.자료종합:①관우조직공정재료여생물체적상호작용:종술료고분자조직공정재료재여생물체조직접촉시발생각충각양적상호작용.지출료재료여생물체적상호작용정황결정료재료조직상용성적정도;재료대조직상용성적영향포함착미관분자수평화굉관척도수평,이차,굉관대척도상(포괄재료표면적탁복결구)적효응비분자척도상발생적화학효응경위중요.②관우재료표면물이화학성질대재료세포상용성적영향:종술료고분자재료적조조표면、다공표면、규칙각조표면、섬유표면、문리결구화단백질층등재료표면적탁복결구대재료세포상용성적영향.지출저충영향시연구조직공정재료적생물상용성급진행조직상용성재료설계적중요내용.결론:재료표면적탁복결구대재료적세포상용성유착교대영향,세포여취합물적상호작용시평개재료세포상용성적지표.세포여고분자재료간단기적상호작용정도가유세포여고분자재료표면적점부정도래평개,이장기적상호작용가이통과검측체외배양세포적생장정황혹재체내식입고분자촌료진행장기평고.
BACKGROUND:To clarify the effects of topology of tissue engineering material surface on cell compatibility from random roughness.porosity,groove/ridge, fiber,texture and protein tracks 6 aspects. STUDY SOURCES: Using the terms"biocompatibility,biocompatibility materials,tissue engineering,tissue engineering materials.cell-compatibility and cell-compatibility materials",we searched the PUBMED database to identify studies published in the English language from January 1987 to January 2007. STUDY SELECTION:The data were selected primarily.The quotations in each paper ware looked for.Inclusive criteria: The contents stated in papers were related with biocompatibility of tissue engineering material. Exclusive criteria: repetitive study or Meta analysis papers.DATA EXTRACTION:Totally 74 related papers were collected.Thirty-two met inclusive criteria,among which,24 related with biocompatibility and 8 related with cell-compatibility materials.The other 42 papers were excluded due to obsolete or repetitive contents. DATA SYNTHESIS:①The interaction of tissue engineering material and living body:Various Interactions producing when high polymer tissue engineering material contacts with tissue of living body are reviewed. It js pointed out that the interaction of material and living body depends on the biocompatibility degree of material;The influences of materials on histocompatibility include microscopic molecular level and macroscopic scale level,moreover,the effect of macroscopic scale level (include the topology of material surface) is more important than chemical effect of microscopic molecular level.②Effects of physical and chemical properties on cell-compatibility of materials:The effects of topology of surface of random roughness,porosity,groove/ridge,fiber, texture and protein tracks 6 kinds of materials on cell-compatibility are reviewed. It is also pointed out that the influences are very important in studying the biocompatibility of tissue engineering material and designing tissue compatibility materials.CONCLUSION:Topology of material surface has great influences on cell-compatibility of material.The interaction of cells and polymer is an index to evaluate cell-compatibility of material.Short-term interaction degree of cell and polymeric material can be assessed by detecting the adhesion degree of cells and polymeric material surface,while long-term interaction by detecting the growth of cells cultured in vitro or polymeric material implanted in vivo.