CAJ | 학술논문

建立了一种用毛细管电泳间接紫外法同时测定郁金中7种金属离子(K+,Na+,Cu2+,Zn2+,Mn2+,Ca2+和Mg2+)的方法,确定了最佳试验条件.结果表明:在214 nm检测波长时,以咪唑、2-羟基异丁酸和硫酸为背景电解质,可使上述离子在10分钟内达到基线分离.该方法表现出良好的重现性及稳定性,方法的检出限为0.1～0.5 mg/L,对大多数离子来说,迁移时间和峰面积重现性的相对误差分别小于0.4%和5%,方法的线性范围为10-5～10-2 mol/L(R2=0.991～0.998).利用该方法对郁金中的7种离子进行了定量测定.
건립료일충용모세관전영간접자외법동시측정욱금중7충금속리자(K+,Na+,Cu2+,Zn2+,Mn2+,Ca2+화Mg2+)적방법,학정료최가시험조건.결과표명:재214 nm검측파장시,이미서、2-간기이정산화류산위배경전해질,가사상술리자재10분종내체도기선분리.해방법표현출량호적중현성급은정성,방법적검출한위0.1～0.5 mg/L,대대다수리자래설,천이시간화봉면적중현성적상대오차분별소우0.4%화5%,방법적선성범위위10-5～10-2 mol/L(R2=0.991～0.998).이용해방법대욱금중적7충리자진행료정량측정.
Yujin (tubers of Curcuma longa) is a type of Chinese medicinal herb which has good effects in invigorating the circulation of blood and preventing cancer and other chronic diseases. The simultaneous determination of K+, Na+, Cu2+, Zn2+,Mn2+, Ca2 + and Mg2 + by capillary electrophoresis with indirect UV detection was studied in a background electrolyte system composed of imidazole, 2-hydroxy isobutyric acid (HIBA) and sulfuric acid. All ions were baseline resolved within 10 min. The applicability of this method was tested by investigations of reproducibility and linearity of the calibration. The detection limit is in the range 0.1 ～ 0.5 mg/L. The reproducibility is less than 0.4% for migration time and less than 5% for the peak area of most inorganic cations. The calibration graphs are linear for most ions in the concentration range of 10- 5 ～ 10-2 mol/L ( R2 = 0.991 ～0. 998). The quantitative determination of the seven ions in Yujin was well carried out by using the developed method.