解剖学报
解剖學報
해부학보
ACTA ANATOMICA SINICA
2008年
5期
693-698
,共6页
马建敏%和俊涛%索世英%宁黔冀%徐存栓
馬建敏%和俊濤%索世英%寧黔冀%徐存栓
마건민%화준도%색세영%저검기%서존전
皮质酮%肝再生%鸟氨酸脱羧酶%RT-PCR%Western blotting%高效液相色谱法%大鼠
皮質酮%肝再生%鳥氨痠脫羧酶%RT-PCR%Western blotting%高效液相色譜法%大鼠
피질동%간재생%조안산탈최매%RT-PCR%Western blotting%고효액상색보법%대서
Corticosterone%Liver regeneration%Ornithine decarboxylase%RT-PCR%Western blotting%High performanceliquid chromatography%Rat
目的 研究大鼠体内主要的糖皮质激素--皮质酮对部分肝切除(PH)诱导的再生肝鸟氨酸脱羧酶(ODC)基因表达及酶活性的影响.方法 乙醚麻醉大鼠,于PH前3d行双侧肾上腺切除术及假手术,皮质酮悬浮于芝麻油中皮下注射玄肾上腺鼠.用RT-PCR、Western blotting及高效液相色谱法(HPLC)分别测定ODC mRNA、ODC蛋白及酶活性.结果 与再生肝相比,完整肝中ODC mRNA、蛋白及其酶活性水平较低.PH后所有组(每组n=6)中ODCmRNA水平均显著升高,5h达到峰值.7h前mRNA含量由高到低依次为去肾上腺组、去肾上腺假手术组(即对照组)、10和40ms/kg体重皮质酮处理组.去肾上腺组(n=13,下同)的ODC蛋白量高于对照组.10mg/kg体重皮质酮处理,在PH后0～24h下降,40mg/kg皮质酬处理组最低.PH后,所有组(每组n=6)ODC活性迅速升高,6h达到峰值;皮质酮处理后活性在6h下降显著,40ms/kg体重皮质酮处理组活性最低.结论 皮质酮处理后,大鼠完整肝及再生肝中ODC mRNA及蛋白水平呈剂量依赖性下降;ODC活性变化的部分原因是由于ODC mRNA及其蛋白量的改变.
目的 研究大鼠體內主要的糖皮質激素--皮質酮對部分肝切除(PH)誘導的再生肝鳥氨痠脫羧酶(ODC)基因錶達及酶活性的影響.方法 乙醚痳醉大鼠,于PH前3d行雙側腎上腺切除術及假手術,皮質酮懸浮于芝痳油中皮下註射玄腎上腺鼠.用RT-PCR、Western blotting及高效液相色譜法(HPLC)分彆測定ODC mRNA、ODC蛋白及酶活性.結果 與再生肝相比,完整肝中ODC mRNA、蛋白及其酶活性水平較低.PH後所有組(每組n=6)中ODCmRNA水平均顯著升高,5h達到峰值.7h前mRNA含量由高到低依次為去腎上腺組、去腎上腺假手術組(即對照組)、10和40ms/kg體重皮質酮處理組.去腎上腺組(n=13,下同)的ODC蛋白量高于對照組.10mg/kg體重皮質酮處理,在PH後0～24h下降,40mg/kg皮質酬處理組最低.PH後,所有組(每組n=6)ODC活性迅速升高,6h達到峰值;皮質酮處理後活性在6h下降顯著,40ms/kg體重皮質酮處理組活性最低.結論 皮質酮處理後,大鼠完整肝及再生肝中ODC mRNA及蛋白水平呈劑量依賴性下降;ODC活性變化的部分原因是由于ODC mRNA及其蛋白量的改變.
목적 연구대서체내주요적당피질격소--피질동대부분간절제(PH)유도적재생간조안산탈최매(ODC)기인표체급매활성적영향.방법 을미마취대서,우PH전3d행쌍측신상선절제술급가수술,피질동현부우지마유중피하주사현신상선서.용RT-PCR、Western blotting급고효액상색보법(HPLC)분별측정ODC mRNA、ODC단백급매활성.결과 여재생간상비,완정간중ODC mRNA、단백급기매활성수평교저.PH후소유조(매조n=6)중ODCmRNA수평균현저승고,5h체도봉치.7h전mRNA함량유고도저의차위거신상선조、거신상선가수술조(즉대조조)、10화40ms/kg체중피질동처리조.거신상선조(n=13,하동)적ODC단백량고우대조조.10mg/kg체중피질동처리,재PH후0～24h하강,40mg/kg피질수처리조최저.PH후,소유조(매조n=6)ODC활성신속승고,6h체도봉치;피질동처리후활성재6h하강현저,40ms/kg체중피질동처리조활성최저.결론 피질동처리후,대서완정간급재생간중ODC mRNA급단백수평정제량의뢰성하강;ODC활성변화적부분원인시유우ODC mRNA급기단백량적개변.
Objective The regulation of ornithine decarboxylase (ODC) gene expression and enzyme activity by corticosterone, the main glucocorticoid in rat, during rat liver regeneration induced by partial hepatectomy (PH) was evaluated.Methods Bilateral adrenaleetomies (ADX) and sham-ADX were performed on ether-anesthetized rats 3 days before PH.Corticosterone in sesame oil was injected subcutaneously to adrenalectomied rats. ODC mRNA, ODC protein and enzyme activity were detected by RT-PCR, Western blotting and high performance liquid chromatography (HPLC), respectively. Results The ODC mRNA levels, protein accumulation and enzyme activity were lower in the intact liver compared to the regenerating liver.After PH, mRNA levels were remarkably enhanced in all groups (n=6 in each group) and peaked at 5 hours post-PH. Till 7 hours, the contents in all groups from high to low were ADX group,control group (Sham-ADX group), ADX treated with 10mg/kg and 40mg/kg body weight corticosterone group, respectively. ODC protein accumulation in ADX rats was higher than that in control rats (n=13, the same below), but it decreasod in corticosterone-treated (10mg/kg) rats until 24 hours post-PH, with a strong decline seen in 40mg/kg corticosterone-treated rats. ODC activity was rapidly promoted, and the highest levels were observed at 6 hours after PH in all groups (n=6 in each group). After corticosterone treatment, the activities declined significantly at 6 hours post-PH, with the lowest value found in the 40mg/kg group. Conclusion Corticosterone treatment results in dose-dependent decreases in ODC mRNA and enzyme protein both in the intact liver and the regenerating liver. The change in ODC activity is partially related to alterations of ODC mRNA and protein accumulation.
