CAJ | 학술논문

异色瓢虫Harmonia axyridis(Pallas)在亚洲地区是一种重要的捕食性天敌昆虫,但是当引入到欧美后,对当地的物种产生了威胁.本文用ISSR技术对两个中国本地种群和一个引入美国的种群进行了种群多样性以及种群分化的分析.8条ISSR引物共扩增出105条带,其中多态性条带比率为96.19%.Nei's多样性指数、Shannon信息指数、多态性条带比率,在中国种群与美国种群中均非常接近,种群分化不明显(G_(st)=0.042).AMOVA分析显示种群多样性主要来源于种群内部,种群问的基因流非常强(N_m=5.537).基于Nei's多样性构建的聚类结果显示ISSR是一种快速有效的研究异色瓢虫种群多样性关系的技术.
이색표충Harmonia axyridis(Pallas)재아주지구시일충중요적포식성천활곤충,단시당인입도구미후,대당지적물충산생료위협.본문용ISSR기술대량개중국본지충군화일개인입미국적충군진행료충군다양성이급충군분화적분석.8조ISSR인물공확증출105조대,기중다태성조대비솔위96.19%.Nei's다양성지수、Shannon신식지수、다태성조대비솔,재중국충군여미국충군중균비상접근,충군분화불명현(G_(st)=0.042).AMOVA분석현시충군다양성주요래원우충군내부,충군문적기인류비상강(N_m=5.537).기우Nei's다양성구건적취류결과현시ISSR시일충쾌속유효적연구이색표충충군다양성관계적기술.
The multicoloured Asian ladybeetle, Harmonia axyridis ( Pallas) , is a well-known aphid predator in its native Asian range and appears a threat to native species after imported into America and Europe. In this paper eight different ISSR primers have been used for analyzing population differentiation and genetic variations among one introduced population in America and two native Chinese populations. The resulting 105 loci showed a total polymorphism of 96. 19%. Genetic diversity estimated by Nei's gene diversity (He) , Shannon's information index (I) and the percentage of polymorphism loci are similar in native and introduced populations. There is no obvious differentiation estimated by Nei's index ( G_(st)= 0.0432) among populations. Most of the genetic variation is observed within populations (AMOVA: 91. 22% and mean polymorphism of 94% ) and very high rates of gene flow were detected (N_m =5. 537) among populations. Phylogenetic dendrogram constructed based on Nei's genetic diversities suggested that ISSR is a quick and effective technique to study the relationship of geographic populations of H. axyridis.