CAJ | 학술논문

采用分光光度法,以盐酸小檗碱为标准品测定新疆红果桑中的总生物碱含量.在提取过程中通过单因素试验分析乙醇体积分数、料液比、提取时间及提取温度4个主要因素对提取率的影响.在单因素试验的基础上通过正交设计法进行试验,优化红果桑总生物碱提取工艺条件.结果表明:红果桑总生物碱的最佳提取工艺条件为乙醇体积分数90%、料液比1:15、提取温度70℃、提取时间6h.根据最佳提取工艺条件测得的红果桑的总生物碱提取率为2.5%.该实验结果可靠,方法简便,最佳条件适合批量生产中该药材的提取.
채용분광광도법,이염산소벽감위표준품측정신강홍과상중적총생물감함량.재제취과정중통과단인소시험분석을순체적분수、료액비、제취시간급제취온도4개주요인소대제취솔적영향.재단인소시험적기출상통과정교설계법진행시험,우화홍과상총생물감제취공예조건.결과표명:홍과상총생물감적최가제취공예조건위을순체적분수90%、료액비1:15、제취온도70℃、제취시간6h.근거최가제취공예조건측득적홍과상적총생물감제취솔위2.5%.해실험결과가고,방법간편,최가조건괄합비량생산중해약재적제취.
Alkaloids in red mulberry (Morus rubra L.) were extracted with aqueous ethanol, and quantified using spectropho-tometry method and berberine hydrochloride as standard. Effects of ethanol concentration, sofid-to-liquid ratio, extraction time and temperature on the alkaloids extraction ratio were studied and optimized by conducting orthogonal test. The optimum extracting conditions were that the red mulberry was extracted with 90% aqueous ethanol and extracted at 70 ℃ for 6 h with the solid-to-liquid ratio at 1:15. Under such conditions, the extraction yield of alkaloids was 2.5%.