CAJ | 학술논문

设置7个萃取温度(25,35,45,55,65,75,85℃)和萃取时间(30,40,50,60,70,80,90 min)来选择超声提取紫椴花中多糖的最佳提取温度和提取时间.利用分光光度法对紫椴花中的多糖含量进行了分离和测定.结果表明:最佳提取条件为提取温度为75℃,提取时间为52 min.经蒽酮-硫酸显色,于580 nm处测定,其多糖的含量为9.74%,RSD=0.47%(n=3).此测定方法简便,样品溶液在4 h内显色稳定,重现性较好,平均回收率为99.48%±1.01%,RSD=0.112%(n=3).
설치7개췌취온도(25,35,45,55,65,75,85℃)화췌취시간(30,40,50,60,70,80,90 min)래선택초성제취자단화중다당적최가제취온도화제취시간.이용분광광도법대자단화중적다당함량진행료분리화측정.결과표명:최가제취조건위제취온도위75℃,제취시간위52 min.경은동-류산현색,우580 nm처측정,기다당적함량위9.74%,RSD=0.47%(n=3).차측정방법간편,양품용액재4 h내현색은정,중현성교호,평균회수솔위99.48%±1.01%,RSD=0.112%(n=3).
Seven extracting temperatures (25, 35, 45, 55, 65, 75, 85℃) and extracting time (30, 40, 50, 60, 70, 80, 90 min) were designed for selecting the optimal extracting time and extracting temperature for ultrasonic extraction of T. amurensis flowers. Polysaccharides from T. amurensis flowers were isolated and determined by spectrophotometry. Results show that the optimal ultrasonic temperature was 75℃ and extracting time 52 min. The content of polysaccharides in T. amurensis flowers measured by anthrone－H_2SO_4 colorimetry under 580 nm, was 9.74% with 0.47% of relative standard deviation (RSD, n=3). This study demonstrated that ultrasonic extraction method was simple, and the color of the treated samples was stable in 4 h. The average recovery value for the polysaccharides measured was 99.48%±1.01%, with 0.112% of RSD (n=3).