CAJ | 학술논문

目的制备紫杉醇纳米粒子,并考察其在实验兔体内经DispatchTM球囊灌注后组织分布情况.方法以生物可降解材料聚乳酸聚乙醇酸共聚物(PLGA)为原料,采用超声乳化-溶剂挥发法制备载紫杉醇纳米粒子.对纳米粒子的粒径、形态、药物含量和体内外释放进行测定.通过新西兰兔腹主动脉局部给药模型考察紫杉醇纳米粒子球囊灌注后组织分布情况.结果制备的紫杉醇纳米粒子的平均粒径约为246 nm,包封率为93.25％,紫杉醇含量19.06％.体外可维持恒定释放30d以上.新西兰兔体内经腹主动脉实现DispatchTM球囊灌注,观察药物可在靶部位体内贮留长达21d.结论紫杉醇PLGA纳米粒子作为一种局部药物传递系统,经球囊灌注在动物模型体内提高局部药物浓度,延长药物作用时间,可实现缓释靶向治疗.
목적 제비자삼순납미입자,병고찰기재실험토체내경DispatchTM구낭관주후조직분포정황.방법 이생물가강해재료취유산취을순산공취물(PLGA)위원료,채용초성유화-용제휘발법제비재자삼순납미입자.대납미입자적립경、형태、약물함량화체내외석방진행측정.통과신서란토복주동맥국부급약모형고찰자삼순납미입자구낭관주후조직분포정황.결과 제비적자삼순납미입자적평균립경약위246 nm,포봉솔위93.25％,자삼순함량19.06％.체외가유지항정석방30d이상.신서란토체내경복주동맥실현DispatchTM구낭관주,관찰약물가재파부위체내저류장체21d.결론 자삼순PLGA납미입자작위일충국부약물전체계통,경구낭관주재동물모형체내제고국부약물농도,연장약물작용시간,가실현완석파향치료.
Objective To prepare paclitaxel-loaded nanoparticles (NPs),and to observe drug biodistribution after intravascular infusion of the NPs using a DispatchTM catheter into New Zealand rabbit abdominal aorta models.Methods Paclitaxel-loaded NPs were prepared by ultrasonication/emulsificcation/solvent evaporation technique using biodegradable poly (lactic-co-glycolic acid)(PLGA) as drug carrier.NP size and morphology was assessed by submicro-laser defractometer and scanning electron microscopy.In vitro release of paclitaxel from the NPs was performed by shaking in PBS at 37℃.The NPs was delivered into New Zealand rabbit abdominal aorta using a DispatchTM catheter.Results The diameter of paclitaxel NPs was around 246 nm with very narrow size distribution.The NPs showed good spherical shape with smooth uniform surface.Paclitaxel loading in the NPs was about 19.06％ with encapsulation efficiency about 93.25％.The NPs maintained a sustained in vitro drug release for 30 days in PBS.After in vivo NP infusion,paclitaxel was detected in the vascular tissue around the infusion site and it retained in the site for 21 days.Conclusion PLGA nanoparticles as local drug delivery carrier showed great potential to maintain a high local drug concentration and prolonged drug resident time in animal model in vivo.