CAJ | 학술논문

目的探讨神经中间丝蛋白NF-66能否作为判断胰岛素瘤良恶性的分子标志物.方法采用免疫组织化学染色法检测胰岛素瘤组织、瘤旁胰腺组织及3株胰岛素瘤细胞株NF-66蛋白的表达,应用单因素及多因素统计分析胰岛素瘤NF-66蛋白表达与肿瘤病理特征及患者预后的相关性.结果 132例胰岛素瘤组织中102例(77％)表达NF-66蛋白,98例瘤旁胰腺组织均未见该蛋白表达(P=4.86×10-31)；3株胰岛素瘤细胞株均高表达NF-66蛋白.118例良性胰岛素瘤中96例(81％)表达NF-66,而14例恶性胰岛素瘤中6例(43％)表达NF-66,恶性胰岛素瘤的表达显著下降(P =0.003).胰岛素瘤NF-66表达与肿瘤大小(以3cm为界)、远处转移、远处并淋巴结转移均呈显著负相关(46％比81％,P=0.009；38％比81％,P=0.013;43％比81％,P=0.010)；而与患者性别、年龄、肿瘤复发和生存状态不相关.结论胰岛素瘤中NF-66表达下调与肿瘤的恶性生物学特征有关,可能成为潜在的鉴别胰岛紊瘤良恶性的分子标志物.
목적 탐토신경중간사단백NF-66능부작위판단이도소류량악성적분자표지물.방법 채용면역조직화학염색법검측이도소류조직、류방이선조직급3주이도소류세포주NF-66단백적표체,응용단인소급다인소통계분석이도소류NF-66단백표체여종류병리특정급환자예후적상관성.결과 132례이도소류조직중102례(77％)표체NF-66단백,98례류방이선조직균미견해단백표체(P=4.86×10-31)；3주이도소류세포주균고표체NF-66단백.118례량성이도소류중96례(81％)표체NF-66,이14례악성이도소류중6례(43％)표체NF-66,악성이도소류적표체현저하강(P =0.003).이도소류NF-66표체여종류대소(이3cm위계)、원처전이、원처병림파결전이균정현저부상관(46％비81％,P=0.009；38％비81％,P=0.013;43％비81％,P=0.010)；이여환자성별、년령、종류복발화생존상태불상관.결론 이도소류중NF-66표체하조여종류적악성생물학특정유관,가능성위잠재적감별이도문류량악성적분자표지물.
Objective To investigate whether neuronal intermediate filament protein(NF-66) could be used as a molecular marker for the determination of malignancy of insulinoma.Methods The expression of NF-66 protein was detected in insulinoma and pana - cancerous tissues and 3 insulinoma cell lines by immunohistochemistry staining. The relationship between the expression of NF-66 protein and the clinicopathological characteristics,survival was analyzed by univariate and multivariate statistical analysis.Results Expression of NF-66 was found in 102(77％ ) out of 132 insulinomas and none of 98 paired control (P =4.86 × 10-31 ).NF-66 was highly expressed in 3 insulinoma cell lines.The expression of NF-66 was found in 96 (81％) out of 118 benign tumors (P =0.003),while out of 14 malignant insulinomas,6(43％ ) were found to express NF-66 ( P =0.003 ).The expression of NF-66 was significantly associated with tumor size (3 cm as the cut-off point),distant metastasis (38％ vs 81％,P =0.013) and distant plus lymph node metastasis (46％ vs 81％,P =0.009),respectively.The expression of NF-66 was not correlated with age,gender,recurrence and overall survival.Conclusions Down-regulation of NF-66 was significantly associated with tumor malignancy,suggesting that NF-66 could be a potentially novel molecular bionarker to distinguish malignancy from benign insulinoma.