CAJ | 학술논문

目的探讨非caspase依赖凋亡途径的凋亡诱导因子(AIF)与caspase依赖凋亡途径的caspase-3在结直肠腺瘤一癌序列中的表达变化及两种蛋白之间的相关性.方法采用免疫组织化学染色检测18例正常黏膜、84例结直肠腺瘤和72例结直肠癌中AIF及caspase-3的表达情况.结果结直肠腺瘤组织中AIF及caspase-3的阳性表达率均明显高于正常黏膜组织(P<0.05);腺瘤组织中AIF阳性表达率与腺癌之间的差异无统计学意义(P>0.05).而caspase-3阳性表达率则明显高于腺癌组织(P<0.05);腺瘤组内绒毛状腺瘤AIF阳性表达率明显低于管状腺瘤(P<0.05),而caspase-3阳性表达率在两种腺瘤之间的差异无统计学意义(P>0.05).相关分析显示,AIF表达与caspase-3表达无显著相关性(P>0.05).结论 AIF代表的非caspasc依赖凋亡途径异常发生在腺瘤向癌突变早期,而caspase依赖途径的凋亡失控可能是更重要的促进结直肠癌发生的因素之一.非caspase依赖的凋亡途径与caspase依赖的凋亡途径是肿瘤发生中两条相对独立的细胞凋亡途径.
목적 탐토비caspase의뢰조망도경적조망유도인자(AIF)여caspase의뢰조망도경적caspase-3재결직장선류일암서렬중적표체변화급량충단백지간적상관성.방법 채용면역조직화학염색검측18례정상점막、84례결직장선류화72례결직장암중AIF급caspase-3적표체정황.결과 결직장선류조직중AIF급caspase-3적양성표체솔균명현고우정상점막조직(P<0.05);선류조직중AIF양성표체솔여선암지간적차이무통계학의의(P>0.05).이caspase-3양성표체솔칙명현고우선암조직(P<0.05);선류조내융모상선류AIF양성표체솔명현저우관상선류(P<0.05),이caspase-3양성표체솔재량충선류지간적차이무통계학의의(P>0.05).상관분석현시,AIF표체여caspase-3표체무현저상관성(P>0.05).결론 AIF대표적비caspasc의뢰조망도경이상발생재선류향암돌변조기,이caspase의뢰도경적조망실공가능시경중요적촉진결직장암발생적인소지일.비caspase의뢰적조망도경여caspase의뢰적조망도경시종류발생중량조상대독립적세포조망도경.
Objective To investigate the expression differenees of apeptosis-indueing factor (AIF) and easpase-3 among coloreetal carcinoma, adenoma and normal mucosa, and to identify the relationship between AIF and caspase-3 expression in coloreetal adenoma-careinoma sequence.Methods Formalin-fixed paraffin embedded colorectal tissues from 174 cases, including 84 adenomas,72 carcinomas, and 18 normal mucosa, were examined for expression of AIF and easpase-3 by streptavidin-peroxidase(SP) immunohistoehemistry. Results The positive rates of AIF and caspase-3 in coloreetal adenoma were higher than those in normal mueosa (P<0.05). The positive rate of AIF in adenoma showed no significant difference compared to coloreetal carcinoma (P>0.05). However, caspase-3 expression in adenomas was significantly higher than that in carcinoma (P<O.05). The positive rate of AIF in tubular adenoma was significantly higher than that in villous adenoma (P<0.05), while the positive expression rate of caspase-3 in the two types of adenoma showed no significant difference (P>0.05). AIF expression had no prominent correlation with the caspase-3 expression (P>0.05). Conclusions The dysregnlation of caspase-independent pathway of apoptosis may be an early event in the development of coloreetal carcinogenesis, while the dysregulation of the easpase-dependent pathway of apoptosis may be one of contributing factors of coloreetal carcinogenesis. The easpase-independent pathway of apoptosis and the caspase-dependent pathway of apoptosis are two relatively independent pathways in eoloreetal carcinogenesis.