CAJ | 학술논문

目的探讨利用高分辨率熔解曲线技术(HRM)检测苯作业人员MDR1 C3435T的可行性.方法首先应用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)检测121例苯作业人员MDR1 3435的基因型,冉随机抽取10%样本进行 HRM分析,在进行HRM的样本中随机抽取6例进行测序验证,并对HRM、PCR-RFLP的优劣性进行比较.结果随机抽取的10%样本经HRM分析后与PCR-RFLP检测的基因型完全一致,经测序证实酶切前PCR产物为244 bp,同时证实MDR1 3435位点存在C/C型、C/T型、T/T型3种基因型,两种方法均可靠.HRM在检测MDR1 C3435T时比RFLP具有耗时短、易于操作、价格便宜的优点.结论 HRM是理想的DNA诊断方法,适合在职业健康监护中筛查易感基因.
목적 탐토이용고분변솔용해곡선기술(HRM)검측분작업인원MDR1 C3435T적가행성.방법 수선응용취합매련반응-한제성편단장도다태성분석(PCR-RFLP)검측121례분작업인원MDR1 3435적기인형,염수궤추취10%양본진행 HRM분석,재진행HRM적양본중수궤추취6례진행측서험증,병대HRM、PCR-RFLP적우렬성진행비교.결과 수궤추취적10%양본경HRM분석후여PCR-RFLP검측적기인형완전일치,경측서증실매절전PCR산물위244 bp,동시증실MDR1 3435위점존재C/C형、C/T형、T/T형3충기인형,량충방법균가고.HRM재검측MDR1 C3435T시비RFLP구유모시단、역우조작、개격편의적우점.결론 HRM시이상적DNA진단방법,괄합재직업건강감호중사사역감기인.
Objective Using high resolution melting ( HRM ) to analysis MDR1 C3435T in people exposed to benzene. Methods Restriction fragment length polymorphism (RFLP) was utilized to detect the polymorphism of MDR1 3435 in 121 benzene-exposed workers, and the results were compared with the HRM in 10% samples and were confirmed with direct sequencing for six people in them. Results By direct sequencing, consistent results of benzene-exposed workers with RFLP or HRM were got. The new high resolution melting curve analysis is more efficient, more convenient, and cheaper than RFLP. Conclusion High-resolution melting analysis provides a valid approach to efficiently detect DNA genetic diagnosis, which is suitable for detect susceptible gens in occupational surveillance.