细胞因子联合对4.5 Gy γ射线照射比格犬造血损伤的治疗作用
세포인자연합대4.5 Gy γ사선조사비격견조혈손상적치료작용
Therapeutic effects of combined cytokines on hematopoietic injuries induced by 4.5 Gy γ-rays irradiation in beagles
  • 万方数据
    中华放射医学与防护杂志 중화방사의학여방호잡지
    Chinese Journal of Radiological Medicine and Protection
    2010年 3期 247-251 ,共5页

    赵建治%李明%邢爽%胡志清%熊国林%谢玲%欧红玲%黄海潇%赵振虎%王宁%王金香%缪竞诚%朱南康%张学光%从玉文%张日%罗庆良

    조건치%리명%형상%호지청%웅국림%사령%구홍령%황해소%조진호%왕저%왕금향%무경성%주남강%장학광%종옥문%장일%라경량

    重组粒细胞集落刺激因子%重组白细胞介素-11%重组白细胞介素-2%造血损伤%比格犬 重組粒細胞集落刺激因子%重組白細胞介素-11%重組白細胞介素-2%造血損傷%比格犬
    중조립세포집락자격인자%중조백세포개소-11%중조백세포개소-2%조혈손상%비격견
    rhG-CSF%rhIL-11%rhIL-2%Hematopoietic injuries%Beagles
    目的 观察细胞因子组合对4.5 Gy γ射线照射比格犬造血系统损伤的治疗效果,为极重度骨髓型急性放射病的临床救治提供实验依据.方法 16只比格犬均给予4.5 Gy60 Co γ射线全身照射,随机分为照射对照、综合对症和细胞因子3组.细胞因子组动物在综合对症支持治疗的基础上应用rhG-CSF、rhIL-11和rhIL-2联合治疗.2 d检测1次外周血象,分别于照射前4 d,照射后1和45 d收集骨髓和外周血进行造血细胞集落培养,制备胸骨病理切片观察组织形态学改变.结果 照射后各组动物外周血各类细胞数急剧下降,细胞因子联合治疗可提高白细胞最低值(1.04 x 109/L,而照射对照组和综合对症组分别为0.28×109/L和0.68×109/L),缩短血小板减少持续时间(细胞因子组24 d,综合对症组33 d),使红细胞维持在正常值范围;照射后1 d骨髓及外周血中造血干细胞集落形成率明显下降,照射后45 d 2个治疗组造血干细胞集落数均恢复为照射前水平;照射对照组动物骨髓造血细胞完全消失,细胞因子治疗使得骨髓造血功能完全恢复,与照射前水平比较差异无统计学意义.结论 rhG-CSF、rhIL-11和rhIL-2联合应用可提高极期时外周血白细胞最低值,加速白细胞、血小板和红细胞恢复,促进4.5 Gy γ射线照射犬体内残留造血干/祖细胞的增殖、分化和成熟,从而加速造血功能的重建.rhG-CSF、rhIL-11和rhIL-2不失为治疗极重度骨髓型急性放射病的有效措施.
    목적 관찰세포인자조합대4.5 Gy γ사선조사비격견조혈계통손상적치료효과,위겁중도골수형급성방사병적림상구치제공실험의거.방법 16지비격견균급여4.5 Gy60 Co γ사선전신조사,수궤분위조사대조、종합대증화세포인자3조.세포인자조동물재종합대증지지치료적기출상응용rhG-CSF、rhIL-11화rhIL-2연합치료.2 d검측1차외주혈상,분별우조사전4 d,조사후1화45 d수집골수화외주혈진행조혈세포집락배양,제비흉골병리절편관찰조직형태학개변.결과 조사후각조동물외주혈각류세포수급극하강,세포인자연합치료가제고백세포최저치(1.04 x 109/L,이조사대조조화종합대증조분별위0.28×109/L화0.68×109/L),축단혈소판감소지속시간(세포인자조24 d,종합대증조33 d),사홍세포유지재정상치범위;조사후1 d골수급외주혈중조혈간세포집락형성솔명현하강,조사후45 d 2개치료조조혈간세포집락수균회복위조사전수평;조사대조조동물골수조혈세포완전소실,세포인자치료사득골수조혈공능완전회복,여조사전수평비교차이무통계학의의.결론 rhG-CSF、rhIL-11화rhIL-2연합응용가제고겁기시외주혈백세포최저치,가속백세포、혈소판화홍세포회복,촉진4.5 Gy γ사선조사견체내잔류조혈간/조세포적증식、분화화성숙,종이가속조혈공능적중건.rhG-CSF、rhIL-11화rhIL-2불실위치료겁중도골수형급성방사병적유효조시.
    Objectivc To observe the therapeutic effects of combined cytokines on hematopoietic injuries induced by 4.5 Gy60 Co γ-rays irradiation in beagles,and to provide experimental evidences for the clinical treatment of extremely severe myeloid acute radiation sickness(ARS).Methods 16 beagles were given 4.5 Gy60 Co γ-rays total body irradiation,and then randomly assigned into irradiation control group,supportive care group and cytokines group.In addition to supportive care,recombinant human granulocyte colony-stimulating factor (rhG-CSF),recombinant human interleukin-11(rhIL-11)and recombinant human interleukin-2(rhIL-2)were administered subcutaneouly to dogs in cytokines group.Peripheral blood hemogram was examined once every two days.Bone marrow and peripheral blood were collected to proceed colony cultivation 4 d pre-irradiation and 1 and 45 d post-irradiation.Conventional histopathological sections of sternum were prepared to observe the histomorphology changes. Results After irradiation,the population of all kinds of cells in peripheral blood declined sharply.WBC nadir Was elevated(1.04×109/L,but 0.28×109/L and 0.68×109/L for the irradiation control group and the supportive care group separately),the duration of thrombocytopenia was shortened (24 days,but 33 days for the supportive care groug) and red blood cell counts were maintained in the range of normal values after cytokincs treatment in combination.The colony forming efficiency of haemopoietic stem cells(HSCs)in bone marrow and peripheral blood decreased obviously 1 d post irradiation,but recovered to the level of that before irradiation 45 d post irradiation after supportive care and cytokines treatment.Hematopoietic cells disappeared in bone marrow of animals in irradiation control group,but hematopoietic functions were recovered after cytokines were administrated.Conclusions RhG-CSF.rhIL-11 and rhIL-2 used in combination could elevate WBC nadir,accelerate the recovery of leukocytes,platelets and red blood cells and promote the proliferation,differentiation and maturity of HSPCs left in the body after 4.5 Gy γ-rays total body irradiation,eventually restore the hematopoietic function.Hence,combination of rhG-CSF,rhIL-11 and rhIL-2 could serve as better therapeutic strategy to treat extremely severe myeloid ARS.
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