中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2010年
6期
586-588
,共3页
克雷伯菌,肺炎%喹诺酮类%抗药性,细菌%膜孔蛋白
剋雷伯菌,肺炎%喹諾酮類%抗藥性,細菌%膜孔蛋白
극뢰백균,폐염%규낙동류%항약성,세균%막공단백
Klebsiella pneumoniae%Quinolones%Drug resistance,bacterial%Porin
目的 探讨氟喹诺酮体外诱导耐药肺炎克雷伯菌(KPn)膜孔蛋白表达变化.方法 取2008年9月至2009年6月本院临床分离对环丙沙星敏感的KPn 20株,分为对照组和实验组,每组10株.对照组直接涂布于含环丙沙星128 mg/L的平板,观察其对环丙沙星的敏感性.实验组应用不同浓度梯度环丙沙星逐级诱导成为高度耐药株,观察应用环丙沙星前后KPn敏感株和耐药株膜孔蛋白表达的差异.结果 对照组10株KPn直接涂布于含环丙沙星128 mg/L的平板培养后无一存活.3对KPn菌株R9、S9、R4、S4、R3、S3环丙沙星诱导前后膜孔蛋白相对表达量分别是3.86±0.11、6.44±0.26、5.46±0.18、9.58±0.34、1.75±0.06和9.78±0.36,诱导耐药的KPn较相应敏感株膜孔蛋白表达明显减少(均P＜0.05).结论 氟喹诺酮体外诱导耐药KPn膜孔蛋白表达减少,可能通过细菌外膜通透性改变在KPn诱导耐药中起重要作用.
目的 探討氟喹諾酮體外誘導耐藥肺炎剋雷伯菌(KPn)膜孔蛋白錶達變化.方法 取2008年9月至2009年6月本院臨床分離對環丙沙星敏感的KPn 20株,分為對照組和實驗組,每組10株.對照組直接塗佈于含環丙沙星128 mg/L的平闆,觀察其對環丙沙星的敏感性.實驗組應用不同濃度梯度環丙沙星逐級誘導成為高度耐藥株,觀察應用環丙沙星前後KPn敏感株和耐藥株膜孔蛋白錶達的差異.結果 對照組10株KPn直接塗佈于含環丙沙星128 mg/L的平闆培養後無一存活.3對KPn菌株R9、S9、R4、S4、R3、S3環丙沙星誘導前後膜孔蛋白相對錶達量分彆是3.86±0.11、6.44±0.26、5.46±0.18、9.58±0.34、1.75±0.06和9.78±0.36,誘導耐藥的KPn較相應敏感株膜孔蛋白錶達明顯減少(均P＜0.05).結論 氟喹諾酮體外誘導耐藥KPn膜孔蛋白錶達減少,可能通過細菌外膜通透性改變在KPn誘導耐藥中起重要作用.
목적 탐토불규낙동체외유도내약폐염극뢰백균(KPn)막공단백표체변화.방법 취2008년9월지2009년6월본원림상분리대배병사성민감적KPn 20주,분위대조조화실험조,매조10주.대조조직접도포우함배병사성128 mg/L적평판,관찰기대배병사성적민감성.실험조응용불동농도제도배병사성축급유도성위고도내약주,관찰응용배병사성전후KPn민감주화내약주막공단백표체적차이.결과 대조조10주KPn직접도포우함배병사성128 mg/L적평판배양후무일존활.3대KPn균주R9、S9、R4、S4、R3、S3배병사성유도전후막공단백상대표체량분별시3.86±0.11、6.44±0.26、5.46±0.18、9.58±0.34、1.75±0.06화9.78±0.36,유도내약적KPn교상응민감주막공단백표체명현감소(균P＜0.05).결론 불규낙동체외유도내약KPn막공단백표체감소,가능통과세균외막통투성개변재KPn유도내약중기중요작용.
Objective To investigate the altered expression of porins in drug-resistant Klebsiella pneumoniae (KPn) induced by fluoroquinolone (FQL) in vitro. Methods Between September 2008 and June 2009, 20 clinical stains of FQL sensitive KPn isolated in our hospital were allocated into control and experimental groups (n=10 each). In control group, KPn strains were directly innoculated on a cultureplate containing 128 mg/L FQL and observed for their sensitivity to FQL. In experimental group, KPn strains were induced into highly drug-resistant strains by different concentration gradients of FQL. The altered expression of porins was then observed in sensitive and drug- resistant strains of KPn before and after using FQL.Results No KPn strains in the control group survived on the cultureplate with 128 mg/L FQL. The relative expressions of porin in 3 pairs of KPn strains (R9 vs S9, R4 vs S4 and R3 vs S3 ) were 3.86±0.11 vs 6.44±0.26, 5.46±0.18 vs 9.58±0.34 and 1.75±0.06 vs 9.78±0.36 before and after FQL induction, respectively. The porin expression in drug-resistant strains was significantly lowered compared with sensitive strains (all P＜0.05). Conclusion The down-regulated expression of porin in FQL-induced drug-resistant KPn strains in vitro and hence lowered permeability of the bacterial outer membrane may play an important role in development of drug-resistance.
