CAJ | 학술논문

目的结合G-显带核型分析诊断染色体易位，并对G显带技术难以鉴定的微小易位进行分析。方法采用生物素标记的显微切割制备的X、Y、14q、10号染色体特异性探针，与患者外周血培养淋巴细胞中期染色体进行荧光原位杂交。结果室温存放近10年的标本、-80℃冻存标本及新鲜标本均可看到清晰的杂交信号，染色体结构异常很清楚。结论染色体涂染技术结合G显带核型分析，可以准确识别G显带技术难以鉴定的染色体微小易位。
목적결합G-현대핵형분석진단염색체역위，병대G현대기술난이감정적미소역위진행분석。방법채용생물소표기적현미절할제비적X、Y、14q、10호염색체특이성탐침，여환자외주혈배양림파세포중기염색체진행형광원위잡교。결과실온존방근10년적표본、-80℃동존표본급신선표본균가간도청석적잡교신호，염색체결구이상흔청초。결론염색체도염기술결합G현대핵형분석，가이준학식별G현대기술난이감정적염색체미소역위。
Objective　 This study was aimed at using chromosome painting technique to detect translocation, especially microtranslocations, on chromosomes in comparison with G-banding analysis. Methods　 Chromosome painting technique was applied to analysis of metaphase chromosomes of patients for detecting translocations with biotin-labeled chromosomes X, Y, 14q, 10 specific probes. Results Fluorescence in situ hybridization FISH signals were shown clearly in slides even in specimen stored at room temperature for 10 years and at -80℃. Translocations were located precisely. Conclusion Microtranslocations, which are hard to analyze by G-banding, can be detected exactly using chromosome painting technique with G-band karyotype on metaphase chromosome.