CAJ | 학술논문

目的为了对铜绿假单胞菌引起的慢性呼吸道感染性疾病的研究,需要真实客观地反映在体内环境综合影响下该菌的致病情况,为此建立了慢性铜绿假单胞菌的肺炎动物模型,并对其引起的相关炎症反应进行分析.方法用富含铜绿假单胞菌的琼脂糖珠灌注小鼠,建立铜绿假单胞慢性感染的动物模型,通过检测肺泡灌注液(BALF)中白细胞数、中性粒细胞百分比、白细胞介素,血清中核基质蛋白MMP-2浓度及肺组织的病理切片观测其炎症反应情况.结果感染后动物模型肺部检出铜绿假单胞菌并出现病理改变,肺部感染各项炎症指标在2～3 d达到峰值,大致7 d恢复正常;铜绿假单胞菌肺炎模型小鼠循环系统中检测MMP-2升高,说明该炎症反应在一定程度上可引起肺部纤维化.结论本实验成功建立了慢性铜绿假单胞菌的肺炎动物模型,可在此基础上对该菌的致病性及耐药性进行进一步研究.
목적 위료대동록가단포균인기적만성호흡도감염성질병적연구,수요진실객관지반영재체내배경종합영향하해균적치병정황,위차건립료만성동록가단포균적폐염동물모형,병대기인기적상관염증반응진행분석.방법 용부함동록가단포균적경지당주관주소서,건립동록가단포만성감염적동물모형,통과검측폐포관주액(BALF)중백세포수、중성립세포백분비、백세포개소,혈청중핵기질단백MMP-2농도급폐조직적병리절편관측기염증반응정황.결과 감염후동물모형폐부검출동록가단포균병출현병리개변,폐부감염각항염증지표재2～3 d체도봉치,대치7 d회복정상;동록가단포균폐염모형소서순배계통중검측MMP-2승고,설명해염증반응재일정정도상가인기폐부섬유화.결론 본실험성공건립료만성동록가단포균적폐염동물모형,가재차기출상대해균적치병성급내약성진행진일보연구.
Objective To research the chronic respiratory infectious diseases caused by Pseudomonas aeruginosa need to objectively the reflection of the real environment in the body, so established a mouse model of chronic pulmonary infection with Pseudomonas aeruginosa and analyzed it's inflammatory response. Methods Establishment of chronic bronchial infection mice model that were inoculated with Pseudomonos aeruginosa-laden agarose beads, and analyzed its inflammatory response by detected the cytokines and MMP-2, and a differential cell count and a total leukocytes count were performed as well. Results Pseudomonas aeruginosa had been detected after infection, and there were changes in pathological. Pulmonary inflammation appeared in 1 d and reached near baseline levels by 7 d after inoculation. It was verified that the peak of inflammatory reaction in Pseudomonas aeruginosa infection is in 2-3 d; the mice did have detectable levels of circulating matrix metalloproteinase-2 ( MMP-2 ) after infection with Pseudomonas aeruginosa. MMP-2 concentrations in the blood serum peaked at 3 d after inoculation. It is indicated that Pseudomonas aeruginosa can initiate a certain degree of pulmonary fibrosis on the basis of the pulmonary inflammation. Conclusion In this study, chronic bronchial infection animal model affected by Pseudomonas aeruginosa was established successfully. Base on this animal model, we can do the pathogenicity and drug resistance of Pseudomonas aeruginosa further study.