CAJ | 학술논문

目的探讨胃癌患者外周血中鸟苷酸环化酶C信使RNA(GC-C mRNA)的表达及其与外周血微转移及病情判断的关系.方法实时荧光定量PCR (RFQ-PCR)检测2009年11月至2010年8月在南通大学附属医院住院的60例胃癌患者和门诊21例异型增生、15例肠上皮化生患者及20名健康体检者外周血中GC-C mRNA的表达,同时检测在这60例胃癌患者手术切除胃癌组织中GC-C mRNA的表达.对数据进行Wilcoxon秩和检验、x2检验或Fisher确切概率法分析,外周血和胃癌组织中GC-C mRNA表达的相关性分析采用Spearman秩相关分析.结果在健康体检者外周血中未检测到GC-C mRNA表达,肠上皮化生和异型增生患者外周血中分别检测到2例和3例阳性,胃癌患者外周血中检测到GC-C mRNA高表达,其表达阳性率为48.3％ (29/60),与健康体检者(0)、肠上皮化生(9.5％,2/21)和异型增生(20.0％,3/15)比较,差异均有统计学意义(均P＜0.05),且其表达与Lauren分型、临床分期、分化程度、浸润深度和淋巴结转移等因素均有关(均P＜0.05).相关性分析显示外周血和胃癌组织中GC-C mRNA的表达呈正相关(r=0.4009,P=0.0015).结论胃癌患者外周血中存在GC-C mRNA表达,且其表达与患者病情进展有关,有望成为胃癌预后判断新的标志物.
목적 탐토위암환자외주혈중조감산배화매C신사RNA(GC-C mRNA)적표체급기여외주혈미전이급병정판단적관계.방법 실시형광정량PCR (RFQ-PCR)검측2009년11월지2010년8월재남통대학부속의원주원적60례위암환자화문진21례이형증생、15례장상피화생환자급20명건강체검자외주혈중GC-C mRNA적표체,동시검측재저60례위암환자수술절제위암조직중GC-C mRNA적표체.대수거진행Wilcoxon질화검험、x2검험혹Fisher학절개솔법분석,외주혈화위암조직중GC-C mRNA표체적상관성분석채용Spearman질상관분석.결과 재건강체검자외주혈중미검측도GC-C mRNA표체,장상피화생화이형증생환자외주혈중분별검측도2례화3례양성,위암환자외주혈중검측도GC-C mRNA고표체,기표체양성솔위48.3％ (29/60),여건강체검자(0)、장상피화생(9.5％,2/21)화이형증생(20.0％,3/15)비교,차이균유통계학의의(균P＜0.05),차기표체여Lauren분형、림상분기、분화정도、침윤심도화림파결전이등인소균유관(균P＜0.05).상관성분석현시외주혈화위암조직중GC-C mRNA적표체정정상관(r=0.4009,P=0.0015).결론 위암환자외주혈중존재GC-C mRNA표체,차기표체여환자병정진전유관,유망성위위암예후판단신적표지물.
Objective To detect the expression of GC-C mRNA in peripheral blood of gastric carcinoma patients and determine its potential candidacy as a molecular biological marker for predicting the micrometastasis and determining the status of gastric cancer.Methods GC-C mRNA from peripheral blood samples of gastric carcinoma ( n =60 ),displastia ( n =21 ),intestinal metaplasia ( n =15 ) and healthy cases (n =20) from November 2009 to August 2010 at Affiliated Hospital of Nantong University were assessed by real-time fluorescent quantitative PCR (RFQ-PCR).Results The expressions of GC-C mRNA in peripheral blood from patients with dysplasia,intestinal metaplasia and healthy controls were absent or very low.And a high level of GC-C mRNA was detected in the patients with gastric carcinoma than those with displastia and intestinal metaplasia,and the positive rate were 48.3％ (29/60),9.5％ (2/21),20.0％ (3/15),respectively ( all P ＜ 0.05 ).The levels of GC-C mRNA were significantly correlated with Lauren typing,clinical stage,tumor differentiation degree,depth of invasion and lymph node metastasis ( all P ＜ 0.05 ). The GC-C mRNA expressions were positively correlated in peripheral blood and gastric carcinomas tissues ( r =0.4009,P =0.0015).Conclusions The over-expression of GC-C mRNA is found in peripheral blood from gastric carcinoma patients.Due to its close correlation with clinical stage and lymph node metastasis,it may become a potential prognostic marker of gastric carcinoma.