CAJ | 학술논문

目的探讨富血小板血浆(PRP)是否促进大鼠海绵体神经再生.方法 24只SD雄性大鼠分成假手术组、双侧海绵体神经切断后立即显微缝合组(单纯缝合组)及缝合后立即局部应用组(PfuP应用组),术后3个月通过电刺激观察海绵体内压的变化来反映其勃起功能,随后取海绵体神经干进行甲苯胺蓝染色,观察轴突的变化来反映神经再生情况.结果术后3个月,海绵体神经单纯缝合组最大海绵体内压为(46±8)cmH2O,明显低于假手术组(109±13)cmH2O(P＜0.01);而PRP应用组为(94±13)em H2O,明显高于单纯缝合组(P＜0.01).甲苯胺蓝染色PRP应用组轴突个数为(121±16),明显高于单纯缝合组(70±14)(P＜0.01),但仍低于假手术组(181±21)(P＜0.01).结论局部应用PRP能明显促进受损阴茎海绵体神经的神经再生和勃起功能的恢复.
목적 탐토부혈소판혈장(PRP)시부촉진대서해면체신경재생.방법 24지SD웅성대서분성가수술조、쌍측해면체신경절단후립즉현미봉합조(단순봉합조)급봉합후립즉국부응용조(PfuP응용조),술후3개월통과전자격관찰해면체내압적변화래반영기발기공능,수후취해면체신경간진행갑분알람염색,관찰축돌적변화래반영신경재생정황.결과 술후3개월,해면체신경단순봉합조최대해면체내압위(46±8)cmH2O,명현저우가수술조(109±13)cmH2O(P＜0.01);이PRP응용조위(94±13)em H2O,명현고우단순봉합조(P＜0.01).갑분알람염색PRP응용조축돌개수위(121±16),명현고우단순봉합조(70±14)(P＜0.01),단잉저우가수술조(181±21)(P＜0.01).결론 국부응용PRP능명현촉진수손음경해면체신경적신경재생화발기공능적회복.
Objective To investigate the effect of platelet rich plasma(PRP)on the regeneration of injured cavernous nerve(CN).Methods Blood was collected from the hearts of 6 SD rata to prepare PRP.24 male adult rats were randomly divided into 3 equal groups:pure suture group undergoing bilateral CN transaction and pure suture immediately,PRP group undergoing bilateral CN transaction+suture+PRP 200 μl to the site of suture,and sham operation group.3 months later intracavernous pressure(ICP)was measured by CN electrostimulation and then samples of CN were obrained to undergo pathological examination.Results 3 months later after surgery,the ICP of the pure suture group was(46±8)cm H2O,significantly lower than that of the sham group[(109±13)cm H2O,P＜0.01],and that of the PRP group was(94±13)cm H2O,significantly higher than that of the pure sutured group(P＜0.01),however,still significantly lower than that of the sham operation group(P＜0.05).The number of axons of CN in the PRP group was(121±16),significantly higher than that of the pure sutured group(70±14,P＜0.01);however,still significantly lower than that of the sham operation group(181±21,P＜0.01).Conclusion PRP can promote the regeneration of injured CN and the recovery of erectile function.