肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2009年
11期
721-723,727
,共4页
程秀菊%魏守刚%刘海燕%张永法%许致胜
程秀菊%魏守剛%劉海燕%張永法%許緻勝
정수국%위수강%류해연%장영법%허치성
榄香烯%神经胶质瘤%细胞凋亡
欖香烯%神經膠質瘤%細胞凋亡
람향희%신경효질류%세포조망
Elemene%Glioma%Apoptosis
目的 从细胞水平和分子水平探讨榄香烯对胶质瘤细胞的作用机制.方法 取胶质细胞瘤的U251细胞株做体外培养,四甲基偶氮唑盐比色(MTT)法检测榄香烯对人神经胶质瘤细胞U251的增生抑制作用,流式细胞术(FCM)检测细胞周期、细胞内钙离子(IECa~(2+))含量、Fas、bcl-2、增生细胞核抗原(PCNA)蛋白表达及细胞凋亡.结果 榄香烯能有效抑制胶质瘤细胞U251的恶性增生,MTT法检测榄香烯处理不同时间(24、48、72 h)的半数生长抑制剂量(IC_(50)),24、48、72 h其IC_(50)分别为40.60、38.14、34.35 μg/ml.经榄香烯作用后,细胞周期可阻滞在S期和G2/M期.用异硫氰酸胍荧光素(Annexin V FITC)染色检测发现细胞凋亡率明显增加.榄香烯还可下调PCNA、Fas蛋白的表达,上调细胞内Ca~(2+)(IECa~(2+))含量,对bcl-2蛋白表达无显著影响.结论 榄香烯有显著的抗胶质瘤细胞增生作用,并能通过下调PCNA蛋白的表达,上调细胞内Ca~(2+)含量,诱导细胞凋亡,对Fas表达也有影响,可能与bcl-2基因表达无关.
目的 從細胞水平和分子水平探討欖香烯對膠質瘤細胞的作用機製.方法 取膠質細胞瘤的U251細胞株做體外培養,四甲基偶氮唑鹽比色(MTT)法檢測欖香烯對人神經膠質瘤細胞U251的增生抑製作用,流式細胞術(FCM)檢測細胞週期、細胞內鈣離子(IECa~(2+))含量、Fas、bcl-2、增生細胞覈抗原(PCNA)蛋白錶達及細胞凋亡.結果 欖香烯能有效抑製膠質瘤細胞U251的噁性增生,MTT法檢測欖香烯處理不同時間(24、48、72 h)的半數生長抑製劑量(IC_(50)),24、48、72 h其IC_(50)分彆為40.60、38.14、34.35 μg/ml.經欖香烯作用後,細胞週期可阻滯在S期和G2/M期.用異硫氰痠胍熒光素(Annexin V FITC)染色檢測髮現細胞凋亡率明顯增加.欖香烯還可下調PCNA、Fas蛋白的錶達,上調細胞內Ca~(2+)(IECa~(2+))含量,對bcl-2蛋白錶達無顯著影響.結論 欖香烯有顯著的抗膠質瘤細胞增生作用,併能通過下調PCNA蛋白的錶達,上調細胞內Ca~(2+)含量,誘導細胞凋亡,對Fas錶達也有影響,可能與bcl-2基因錶達無關.
목적 종세포수평화분자수평탐토람향희대효질류세포적작용궤제.방법 취효질세포류적U251세포주주체외배양,사갑기우담서염비색(MTT)법검측람향희대인신경효질류세포U251적증생억제작용,류식세포술(FCM)검측세포주기、세포내개리자(IECa~(2+))함량、Fas、bcl-2、증생세포핵항원(PCNA)단백표체급세포조망.결과 람향희능유효억제효질류세포U251적악성증생,MTT법검측람향희처리불동시간(24、48、72 h)적반수생장억제제량(IC_(50)),24、48、72 h기IC_(50)분별위40.60、38.14、34.35 μg/ml.경람향희작용후,세포주기가조체재S기화G2/M기.용이류청산고형광소(Annexin V FITC)염색검측발현세포조망솔명현증가.람향희환가하조PCNA、Fas단백적표체,상조세포내Ca~(2+)(IECa~(2+))함량,대bcl-2단백표체무현저영향.결론 람향희유현저적항효질류세포증생작용,병능통과하조PCNA단백적표체,상조세포내Ca~(2+)함량,유도세포조망,대Fas표체야유영향,가능여bcl-2기인표체무관.
Objective To explore the activity of Elemene for glioma cell from the cellular and molecular level. Methods The human glioma cell U251 was cultured. The effect of Elemene for human glioma cell proliferation was studied by MTT assay. Cell cycle, Fas, PCNA, bcl-2, intracellular Ca~(2+) and apoptosis were evaluated by flow cytometry analysis. Results Elemene exhibited antiproliferative effect on human glioma cell U251 markedly. The fifty percent inhibition on concentration (IC_(50)) of Elemene against glioma cells at different time points. 24 h was 40.60 μg/ml, the 48 h 38.14 μg/ml and the 72 h 34.35 μg/ml.Cell cycle was blocked in the S and G_2/M phases. The apoptosis ratio was increased by Annexin V staining markedly. Elemene decreased the gene expressions of PCNA and Fas, increased the intracellular Ca~(2+). There was no significant effect on the bcl -2 gene expression. Conclusion Elemene exhibits a marked antiproliferative effect on glioma cells and induces apoptosis by decreasing the expression of PCNA and increasing intracellular Ca~(2+). It also influences the expression of Fas. It might have no relationship with bcl-2 gene expression.
