CAJ | 학술논문

观察粉防己碱对高血压心肌肥厚大鼠心肌胶原含量和肌球蛋白ATP酶活性的影响. 采用二肾一夹肾血管性高血压造成大鼠左心室肥厚模型, 自术后第9周起按粉防己碱50 mg*kg-1, 依那普利6mg*g-1灌胃给药, 每日1次, 连续8周. 用分光光度法测定心肌羟脯氨酸含量及肌球蛋白ATP酶活性. 结果表明左心室肥厚组心肌羟脯氨酸为(5.9±0.3)mg*g-1干重, 明显高于假手术对照组〔(3.6±0.4)mg*g-1干重〕, 粉防己碱组和依那普利组心肌羟脯氨酸含量分别较左心室肥厚组低28.2%和39.0%. 左心室肥厚组肌球蛋白ATP酶活性仅为(0.43±0.09) mmol Pi*min-1*g-1蛋白质, 较假手术对照组〔(0.97±0.06) mmol Pi*min-1*g-1蛋白质〕明显降低, 而粉防己碱组和依那普利组则分别比左心室肥厚组高60.5%和118.6%. 实验结果表明粉防己碱可部分逆转肾血管性高血压所致的大鼠左心室肥厚, 降低心肌胶原含量, 升高肌球蛋白ATP酶活性.
관찰분방기감대고혈압심기비후대서심기효원함량화기구단백ATP매활성적영향. 채용이신일협신혈관성고혈압조성대서좌심실비후모형, 자술후제9주기안분방기감50 mg*kg-1, 의나보리6mg*g-1관위급약, 매일1차, 련속8주. 용분광광도법측정심기간포안산함량급기구단백ATP매활성. 결과표명좌심실비후조심기간포안산위(5.9±0.3)mg*g-1간중, 명현고우가수술대조조〔(3.6±0.4)mg*g-1간중〕, 분방기감조화의나보리조심기간포안산함량분별교좌심실비후조저28.2%화39.0%. 좌심실비후조기구단백ATP매활성부위(0.43±0.09) mmol Pi*min-1*g-1단백질, 교가수술대조조〔(0.97±0.06) mmol Pi*min-1*g-1단백질〕명현강저, 이분방기감조화의나보리조칙분별비좌심실비후조고60.5%화118.6%. 실험결과표명분방기감가부분역전신혈관성고혈압소치적대서좌심실비후, 강저심기효원함량, 승고기구단백ATP매활성.
To study the effects of tetrandrine (Tet) on hypertrophied myocardial hydroxyproline content and myosin ATPase activity, left ventricular hypertrophy(LVH) was induced by renovascular hypertension (two-kidney, one-clip) in rats. Eight weeks after operation Tet 50 mg*kg-1*d-1 and enalapril(Ena) 6 mg*kg-1*d-1 were given by gavage for 8 weeks. The results showed that hydroxyproline content in LVH group was much higher than that of sham-operated one 〔(5.9±0.3) vs (3.6±0.4) mg*g-1 dry weight〕, and was decreased by 28.2% and 39.0% in Tet and Ena groups, respectively. Myosin ATPase activity in LVH group was much lower than that of sham-operated group 〔(0.43±0.09) vs (0.97±0.06) mmol Pi*min-1*g-1 protein, P<0.01〕. In Tet and Ena groups they were 60.5% and 118.6% higher than that of LVH group, respectively. The results suggest that Tet or Ena partially reduce the hydroxyproline content and elevate myosin ATPase activity of hypertrophied myocardium in renovascular hypertensive rats.