CAJ | 학술논문

背景与目的:评价X线放射治疗对鼻咽癌患者外周血淋巴细胞DNA的损伤.材料与方法:采用碱性单细胞凝胶电泳技术,检测19例鼻咽癌X线放射治疗患者在累积照射剂量为0、2、4、8、10、16 Gy时,外周血淋巴细胞的拖尾率,尾长及Olive尾距. 结果:鼻咽癌患者接受X线放射治疗后,淋巴细胞拖尾率、尾长在累积照射剂量为2 Gy时即出现明显上升(P<0.01和P<0.05),Olive尾矩在累积照射剂量为8 Gy时出现明显上升(P<0.01).在0~16 Gy照射剂量范围内随着剂量的升高外周血淋巴细胞的拖尾率,尾长及Olive尾距随之增加,且呈剂量-反应关系.结论:在本实验条件下,X线放射治疗在低剂量时即对鼻咽癌患者外周血淋巴细胞DNA有损伤作用,其照射剂量与外周血淋巴细胞的拖尾率,尾长及Olive尾距呈剂量-反应关系.
배경여목적:평개X선방사치료대비인암환자외주혈림파세포DNA적손상.재료여방법:채용감성단세포응효전영기술,검측19례비인암X선방사치료환자재루적조사제량위0、2、4、8、10、16 Gy시,외주혈림파세포적타미솔,미장급Olive미거. 결과:비인암환자접수X선방사치료후,림파세포타미솔、미장재루적조사제량위2 Gy시즉출현명현상승(P<0.01화P<0.05),Olive미구재루적조사제량위8 Gy시출현명현상승(P<0.01).재0~16 Gy조사제량범위내수착제량적승고외주혈림파세포적타미솔,미장급Olive미거수지증가,차정제량-반응관계.결론:재본실험조건하,X선방사치료재저제량시즉대비인암환자외주혈림파세포DNA유손상작용,기조사제량여외주혈림파세포적타미솔,미장급Olive미거정제량-반응관계.
BACKGROUND AND AIM: To evaluate DNA damage induced by radiotherapy in nasopharyngeal cancer (NPC) patients. MATERIALS AND METHODS: Comet assay was employed to detect comet frequency , tail length and Olive moment of peripheral blood lymphocytes from 19 NPC patients undergoing radiotherapy at the accumulated dose of 0, 2, 4, 8, 10 and 16 Gy. RESULTS: After radiotherapy , the comet frequency and tail length in peripheral lymphocytes rose significantly at the dose of 2 Gy(P<0.01 and P<0.05) whilst Olive moment rose significantly at the dose of 8 Gy(p<0.0l). The comet frequency , tail length and Olive moment all increased following radiation dose in this study (0~ 16 Gy) ,with a clear dose-response relationship. CONCLUSION: Radiotherapy caused DNA damage in NPC patients at low dose, demonstrating dose-effect relationship between radiation dose and comet frequency , tail length and Olive moment.