CAJ | 학술논문

目的:建立用碱性蛋白酶水解罗非鱼的最佳工艺条件.方法:以水解度为优化指标,利用单因素试验考察酶解温度、pH值、反应时间等因素对蛋白水解的影响,利用中心组合试验方法优化酶的添加量和底物蛋白质浓度.结果:碱性蛋白酶水解罗非鱼的最佳条件为加酶量23740U/g蛋白、蛋白质质量分数2.76%、水解温度55℃、pH7.5、水解时间8h,在此条件下,水解体系的氨基酸态氮的含量达到了1.43mg/ml,水解度为33.35%,挥发性盐基氮的含量为0.034mg/ml.
목적:건립용감성단백매수해라비어적최가공예조건.방법:이수해도위우화지표,이용단인소시험고찰매해온도、pH치、반응시간등인소대단백수해적영향,이용중심조합시험방법우화매적첨가량화저물단백질농도.결과:감성단백매수해라비어적최가조건위가매량23740U/g단백、단백질질량분수2.76%、수해온도55℃、pH7.5、수해시간8h,재차조건하,수해체계적안기산태담적함량체도료1.43mg/ml,수해도위33.35%,휘발성염기담적함량위0.034mg/ml.
s : To obtain high degree of hydrolysis (DH) and concentration of amino acid nitrogen, we optimized the hydrolysis catalyzed by alkaline protease of ground whole tilapia. The effects of three crucial variables including temperature, pH and hydrolysis time on the hydrolysis were investigated by single-factor method while the effects of dosage of enzyme and protein concentration were analyzed by response surface methodology (RSM) where the interaction between the two factors was also considered. Under the optimized conditions including dosage of alkaline protease 23740 U/g protein, protein concentration 2.76%, 55 ℃, pH 7.5 for a hydrolysis of 8 h, a concentration of amino acid nitrogen of 1.43 mg/ml and a DH of 33.35% were achieved, and the total volatile basic nitrogen reached 0.034 mg/ml.