CAJ | 학술논문

采用硫酸二乙酯的化学诱变方法,通过对突变株的推理选育筛选得一株乳链菌肽抗性菌株L-D-2,效价最高达到2 570 IU/mL,比出发菌株提高了2.13倍.该菌株传代的稳定性试验表明:L-D-2有较稳定的产乳链菌肽的遗传特性.推理选育乳链菌肽高产菌株的方法快速、有效,可以进一步推广.
채용류산이을지적화학유변방법,통과대돌변주적추리선육사선득일주유련균태항성균주L-D-2,효개최고체도2 570 IU/mL,비출발균주제고료2.13배.해균주전대적은정성시험표명:L-D-2유교은정적산유련균태적유전특성.추리선육유련균태고산균주적방법쾌속、유효,가이진일보추엄.
Applied the way of DES mutation and filter mutation stain through rational screening. The mutant L-D-2 was obtained, which has a productivity with 2570 IU/mL and was 2.13 times higher than the original strain. The experiment results of L-D-2 continuous generations showed that the strain had higher and stable genetic characteristic. The way of rational screening high producing Nisin strain is clipping and effective, which can be more extended.