生物技术
生物技術
생물기술
BIOTECHNOLOGY
2009年
6期
77-78
,共2页
荆士华%汲涌%李春杰%姜铭妍%杨伟超%谌明%徐慧
荊士華%伋湧%李春傑%薑銘妍%楊偉超%諶明%徐慧
형사화%급용%리춘걸%강명연%양위초%심명%서혜
发酵%山梨醇%L-山梨糖%种液
髮酵%山梨醇%L-山梨糖%種液
발효%산리순%L-산리당%충액
fermentation%sorbitol%L-sorbose%seed liquid
目的:调节生黑醋酸杆菌生物和代谢特性,以提高发酵效率.方法:通过改变种液特性,采用半连续培养的方式,对生黑醋酸杆菌在高醇浓度下的生长特性进行了研究.结果:通过优化可以提高VC一步发酵底物山梨醇浓度达38%,32h左右发酵率达95%,山梨糖产量达360mg/ml,半连续培养连续5批之间产糖稳定,没有明显差别.结论:通过优化,有效地提高了山梨糖的产率.
目的:調節生黑醋痠桿菌生物和代謝特性,以提高髮酵效率.方法:通過改變種液特性,採用半連續培養的方式,對生黑醋痠桿菌在高醇濃度下的生長特性進行瞭研究.結果:通過優化可以提高VC一步髮酵底物山梨醇濃度達38%,32h左右髮酵率達95%,山梨糖產量達360mg/ml,半連續培養連續5批之間產糖穩定,沒有明顯差彆.結論:通過優化,有效地提高瞭山梨糖的產率.
목적:조절생흑작산간균생물화대사특성,이제고발효효솔.방법:통과개변충액특성,채용반련속배양적방식,대생흑작산간균재고순농도하적생장특성진행료연구.결과:통과우화가이제고VC일보발효저물산리순농도체38%,32h좌우발효솔체95%,산리당산량체360mg/ml,반련속배양련속5비지간산당은정,몰유명현차별.결론:통과우화,유효지제고료산리당적산솔.
Objective: To control the characteristic of Gluconbacter melanogenus in medium with high concentration of D-sordibol(38%). Method: by changing seed liquid and using semi-continuous fermentation.Result: The strain could be cultured well in medium containing D-sorbitol in 38 % . The conversion rate of D-sorbitol to L-sorbose in medium with 38 % D-sorbitol was 95 % over 30 hours. There was no notably different about the yields of L-sorbose in 5 generations fermentations continuously. Conclusion: The productivity of sorbose was improved notably.
