国际眼科杂志
國際眼科雜誌
국제안과잡지
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2012年
1期
4-6
,共3页
细胞培养%视网膜神经元%胰蛋白酶消化法%神经元特异性烯醇化酶
細胞培養%視網膜神經元%胰蛋白酶消化法%神經元特異性烯醇化酶
세포배양%시망막신경원%이단백매소화법%신경원특이성희순화매
cell culture%retinal neuron%trypsin digestion%neuron specific enolase
目的:在前人建立的方法上优化SD大鼠视网膜神经细胞体外培养的技术和方法,为后续研究提供实验基础.方法:使用胰酶消化法分离新生1～3d SD大鼠视网膜神经细胞,以DMEM/F12为培养基体外培养,免疫组织化学染色的方法进行鉴定.结果:观察光镜下培养的细胞贴壁生长,部分细胞伸出突起,且有些突起相互连接.免疫细胞化学染色显示,培养的细胞大多数抗神经元特异性烯醇化酶(neuron specific enolase,NSE)抗体反应阳性.结论:视网膜神经细胞体外培养成功为进一步进行视网膜疾病的研究创造了条件.
目的:在前人建立的方法上優化SD大鼠視網膜神經細胞體外培養的技術和方法,為後續研究提供實驗基礎.方法:使用胰酶消化法分離新生1～3d SD大鼠視網膜神經細胞,以DMEM/F12為培養基體外培養,免疫組織化學染色的方法進行鑒定.結果:觀察光鏡下培養的細胞貼壁生長,部分細胞伸齣突起,且有些突起相互連接.免疫細胞化學染色顯示,培養的細胞大多數抗神經元特異性烯醇化酶(neuron specific enolase,NSE)抗體反應暘性.結論:視網膜神經細胞體外培養成功為進一步進行視網膜疾病的研究創造瞭條件.
목적:재전인건립적방법상우화SD대서시망막신경세포체외배양적기술화방법,위후속연구제공실험기출.방법:사용이매소화법분리신생1～3d SD대서시망막신경세포,이DMEM/F12위배양기체외배양,면역조직화학염색적방법진행감정.결과:관찰광경하배양적세포첩벽생장,부분세포신출돌기,차유사돌기상호련접.면역세포화학염색현시,배양적세포대다수항신경원특이성희순화매(neuron specific enolase,NSE)항체반응양성.결론:시망막신경세포체외배양성공위진일보진행시망막질병적연구창조료조건.
·AIM: To explore an experimental method for primary culture of retinal neurons of neonatal rat.·METHODS: Retina of postnatal 1-3 days SD rats was dissected into cell suspension by using trypsin digestion and cultured in vitro with DMEM/F12. Immunocytochemical methods were used to identify the cultured neurons.·RESULTS: All cultured cells underwent adherence and some possessed axons，of which some were connected with each other．Most cells were neuron specific enolase(NSE)-positive detected by immunohistochemistry．·CONCLUSION: Successful culture of retinal neuron cells in vitro is helpful in the research of retinal diseases.
