中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2012年
9期
661-666
,共6页
程学美%焦燕妮%陈金东%单宝德%夏昭林
程學美%焦燕妮%陳金東%單寶德%夏昭林
정학미%초연니%진금동%단보덕%하소림
1,3-丁二烯类%生物学标记%色谱法%高压液相
1,3-丁二烯類%生物學標記%色譜法%高壓液相
1,3-정이희류%생물학표기%색보법%고압액상
Butadiene%Biological markers%Chromatography%High pressure liquid
目的 探讨1,3-丁二烯(BD)接触工人的内剂量生物标志物,为BD接触工人生物限值的制定提供基础依据.方法 选择某橡胶厂BD接触工人44名作为接触组,不接触BD的25人为对照组(同一企业锅炉车间工人12名,为内对照；某机构文员13名,为外对照).收集班末尿,用固相微萃取柱对尿液进行处理,UPLC-MS/MS检测,同位素内标定量尿液中BD代谢产物3,4-二羟基硫尿酸(DHBMA)和1-羟甲基-2-苯硫酚尿酸(MHBMA)的浓度,同时用气相色谱-火焰离子化检测器测定工人作业场所空气中BD浓度,比较其内外暴露指标间的相关性.尿中DHBMA和MHBMA及空气中BD测 定数据均经对数转换后统计分析.结果 接触组尿中DHBMA [(2.51 ±0.44) μg/L]和(MHBMA+DHBMA)[(2.68±0.27) μg/L]明显高于对照组[DHBMA:(2.20±0.25) μg/L,(MHBMA+DHBMA):(2.49±0.34) μg/L],差异有统计学意义(P＜0.01)；尿中DHBMA的含量受空气中BD浓度影响明显(r=0.539,P=0.001),回归方程为y=2.417 +0.520x,模型的调整R2为0.262;尿中MHBMA不受到吸烟、饮酒和工龄的影响.结论 在职业环境暴露水平下,班末尿中DHBMA可以作为BD接触的内剂量标志物.
目的 探討1,3-丁二烯(BD)接觸工人的內劑量生物標誌物,為BD接觸工人生物限值的製定提供基礎依據.方法 選擇某橡膠廠BD接觸工人44名作為接觸組,不接觸BD的25人為對照組(同一企業鍋爐車間工人12名,為內對照；某機構文員13名,為外對照).收集班末尿,用固相微萃取柱對尿液進行處理,UPLC-MS/MS檢測,同位素內標定量尿液中BD代謝產物3,4-二羥基硫尿痠(DHBMA)和1-羥甲基-2-苯硫酚尿痠(MHBMA)的濃度,同時用氣相色譜-火燄離子化檢測器測定工人作業場所空氣中BD濃度,比較其內外暴露指標間的相關性.尿中DHBMA和MHBMA及空氣中BD測 定數據均經對數轉換後統計分析.結果 接觸組尿中DHBMA [(2.51 ±0.44) μg/L]和(MHBMA+DHBMA)[(2.68±0.27) μg/L]明顯高于對照組[DHBMA:(2.20±0.25) μg/L,(MHBMA+DHBMA):(2.49±0.34) μg/L],差異有統計學意義(P＜0.01)；尿中DHBMA的含量受空氣中BD濃度影響明顯(r=0.539,P=0.001),迴歸方程為y=2.417 +0.520x,模型的調整R2為0.262;尿中MHBMA不受到吸煙、飲酒和工齡的影響.結論 在職業環境暴露水平下,班末尿中DHBMA可以作為BD接觸的內劑量標誌物.
목적 탐토1,3-정이희(BD)접촉공인적내제량생물표지물,위BD접촉공인생물한치적제정제공기출의거.방법 선택모상효엄BD접촉공인44명작위접촉조,불접촉BD적25인위대조조(동일기업과로차간공인12명,위내대조；모궤구문원13명,위외대조).수집반말뇨,용고상미췌취주대뇨액진행처리,UPLC-MS/MS검측,동위소내표정량뇨액중BD대사산물3,4-이간기류뇨산(DHBMA)화1-간갑기-2-분류분뇨산(MHBMA)적농도,동시용기상색보-화염리자화검측기측정공인작업장소공기중BD농도,비교기내외폭로지표간적상관성.뇨중DHBMA화MHBMA급공기중BD측 정수거균경대수전환후통계분석.결과 접촉조뇨중DHBMA [(2.51 ±0.44) μg/L]화(MHBMA+DHBMA)[(2.68±0.27) μg/L]명현고우대조조[DHBMA:(2.20±0.25) μg/L,(MHBMA+DHBMA):(2.49±0.34) μg/L],차이유통계학의의(P＜0.01)；뇨중DHBMA적함량수공기중BD농도영향명현(r=0.539,P=0.001),회귀방정위y=2.417 +0.520x,모형적조정R2위0.262;뇨중MHBMA불수도흡연、음주화공령적영향.결론 재직업배경폭로수평하,반말뇨중DHBMA가이작위BD접촉적내제량표지물.
Objective To discuss the urine biomarkers in 1,3-butadiene exposed workers,and to provide basement for establishing biological limit value.Methods 44 BD exposed workers as exposure group and 25 BD non-exposed people as control group including 12 workers in boiler workshop in the same factory and 13 people in one public institute,we collected their in-end-of shift urine,then detected urine BD-derived mercapturic metabolites [3,4-dihydroxybutyl mercapturic acid (DHBMA),1-and 2-monohydroxy-3-butenyl mercapturic acid (MHBMA)]concentrations using UPLC-MS/MS method.Meanwhile,we detected air BD concentration with GC-FID in the workplace,and compared their relationship.Results IgDHBMA and lg (MHBMA+DHBMA) levels in exposed group (lgDHBMA:(2.51±0.44) μg/L,lg[MHBMA+DHBMA:(2.68±0.27) μg/L]were higher than which in control group (lgDHBMA:(2.20±0.25) μg/L,lg (MHBMA+DHBMA:(2.49±0.34) μg/L),and the differences were significant (P＜0.01).Urine DHBMA was obviously influenced by air BD concentrations (r=0.539,P=0.001).The equation of Multiple Regression Analysis was y=2.417+0.520x (x represents air BD dose,and y represents urinary DHBMA level).Adjusted R2 of this model was 0.262.Urinary MHBMA was not affected by smoking,alcohol and years of works.Conclusion Urine metabolite DHBMA in BD-exposed workers might be major biological exposure indice.
