CAJ | 학술논문

目的研究表皮生长因子(EGF)对脂肪干细胞体外增殖、迁移和凋亡的影响.方法利用无血清培养基建立体外细胞生长饥饿模型和Fas配体(FasL)诱导的凋亡模型,观察10 nmol/L和100 nmol/L的EGF对脂肪干细胞体外增殖、迁移和凋亡的影响,并检测影响上述过程相关细胞通道蛋白磷脂酶C-γ(PLC-γ)、胞外信号调控激酶(ERK)和丝/苏氨酸激酶(AKT)的表达. 结果10 nmol/L和100 nmol/L的EGF均能有效促进脂肪干细胞体外增殖、迁移和抗凋亡作用,且影响上述过程的相关信号通道蛋白的表达均发生上调.结论 EGF能有效促进脂肪干细胞体外增殖、迁移和抗凋亡.
목적 연구표피생장인자(EGF)대지방간세포체외증식、천이화조망적영향.방법 이용무혈청배양기건입체외세포생장기아모형화Fas배체(FasL)유도적조망모형,관찰10 nmol/L화100 nmol/L적EGF대지방간세포체외증식、천이화조망적영향,병검측영향상술과정상관세포통도단백린지매C-γ(PLC-γ)、포외신호조공격매(ERK)화사/소안산격매(AKT)적표체. 결과10 nmol/L화100 nmol/L적EGF균능유효촉진지방간세포체외증식、천이화항조망작용,차영향상술과정적상관신호통도단백적표체균발생상조.결론 EGF능유효촉진지방간세포체외증식、천이화항조망.
Objective To investigate the effects of epidermal growth factor (EGF) on the proliferation , migration and apoptosis of adipose-derived stem cells (ADSCs) in vitro. Methods Starved cell model and FasL-induced apoptosis model were established in serum-free media. The effects of 10 nmol/L and 100 nmol/L of EGF on the proliferation, migration and apoptosis of ADSCs in vitro were observed. The exrpessions of signal pathway proteins like phospholipaseC-γ(PLC-γ) , extracellular regulated kinase (ERK) and AKT were also detected. Results The proliferation, migration and anti-ap-optosis of ADSCs were promoted by 10 nmol/L or 100 nmol/L of EGF, and the expressions of PLC-'y, ERK and AKT were up-regulated. Conclusion EGF can promote the proliferation, migration and an-ti-apoptosis of adipose-derived ADSCs in vitro.