中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2011年
10期
803-806
,共4页
结肠肿瘤%HCT-116%肿瘤坏死因子相关凋亡诱导配体%凋亡%炎性反应
結腸腫瘤%HCT-116%腫瘤壞死因子相關凋亡誘導配體%凋亡%炎性反應
결장종류%HCT-116%종류배사인자상관조망유도배체%조망%염성반응
Colonic neoplasms%HCT-116%Tumor necrosis factor-related apoptosis-inducing ligand%Apoptosis%Inflammation
目的 探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)对结肠癌细胞凋亡和炎性反应相关基因表达的影响.方法 HCT-116细胞经10 μg/L和100μg/L重组人类TRAIL蛋白处理后,应用荧光实时定量PCR和试剂盒测定凋亡相关基因(Bel-2、Bad、caspase-3和-8)和炎性反应相关基因(TNF-α,IL-1β,COX-2)的表达水平.结果 经10 μg/L和100 μg/L重组人类TRAIL蛋白孵育处理24 h后,HCT-116细胞的凋亡率分别为27.4％和45.9％.同时抗凋亡基因Bcl-2、促凋亡基因Bad以及凋亡指示基因caspase-3和caspase-8的表达均显著上调,且100 μg/L组的上调幅度均高于10μg/L组(P＜0.05).TRAIL蛋白处理后,炎性反应相关基因TNF-α,IL-1β,COX-2的表达亦显著上升,且100 μg/L处理组的上升幅度均高于10μg/L组(P＜0.05).结论 TRAIL重组蛋白能够诱导结肠癌细胞凋亡以及炎性反应的发生.
目的 探討腫瘤壞死因子相關凋亡誘導配體(TRAIL)對結腸癌細胞凋亡和炎性反應相關基因錶達的影響.方法 HCT-116細胞經10 μg/L和100μg/L重組人類TRAIL蛋白處理後,應用熒光實時定量PCR和試劑盒測定凋亡相關基因(Bel-2、Bad、caspase-3和-8)和炎性反應相關基因(TNF-α,IL-1β,COX-2)的錶達水平.結果 經10 μg/L和100 μg/L重組人類TRAIL蛋白孵育處理24 h後,HCT-116細胞的凋亡率分彆為27.4％和45.9％.同時抗凋亡基因Bcl-2、促凋亡基因Bad以及凋亡指示基因caspase-3和caspase-8的錶達均顯著上調,且100 μg/L組的上調幅度均高于10μg/L組(P＜0.05).TRAIL蛋白處理後,炎性反應相關基因TNF-α,IL-1β,COX-2的錶達亦顯著上升,且100 μg/L處理組的上升幅度均高于10μg/L組(P＜0.05).結論 TRAIL重組蛋白能夠誘導結腸癌細胞凋亡以及炎性反應的髮生.
목적 탐토종류배사인자상관조망유도배체(TRAIL)대결장암세포조망화염성반응상관기인표체적영향.방법 HCT-116세포경10 μg/L화100μg/L중조인류TRAIL단백처리후,응용형광실시정량PCR화시제합측정조망상관기인(Bel-2、Bad、caspase-3화-8)화염성반응상관기인(TNF-α,IL-1β,COX-2)적표체수평.결과 경10 μg/L화100 μg/L중조인류TRAIL단백부육처리24 h후,HCT-116세포적조망솔분별위27.4％화45.9％.동시항조망기인Bcl-2、촉조망기인Bad이급조망지시기인caspase-3화caspase-8적표체균현저상조,차100 μg/L조적상조폭도균고우10μg/L조(P＜0.05).TRAIL단백처리후,염성반응상관기인TNF-α,IL-1β,COX-2적표체역현저상승,차100 μg/L처리조적상승폭도균고우10μg/L조(P＜0.05).결론 TRAIL중조단백능구유도결장암세포조망이급염성반응적발생.
Objective To study the effect of recombinant tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on the expression of apoptosis and inflammatory related genes in human colon cancer cell line HCT-116.Methods After 24-hour treatment with recombinant human TRAIL protein,the expressions of apoptosis-related genes (Bcl-2,Bad,caspase-3,and caspase-8) and inflammation-related genes (TNF-α,IL-1β,and COX-2) were measured by real-time PCR and appropriate kits in HCT-116.Results After treatments of 10 μg/L and 100 μg/L recombinant human TRAIL proteins,the apoptotic rates of HCT-116 cells were 27.4％ and 45.9％,respectively.Expressions of anti-apoptosis gene Bcl-2,pro-apoptosis gene Bad and apoptotic markers caspase-3 and caspase-8were significantly up-regulated,which was more significant in the group of 100 μg/L treatment(P＜0.05).Moreover,after TRAIL treatments,expressions of inflammation-related genes TNF-α,IL-1,COX-2were also dramatically increased,and 100 μg/L treatment group showed higher up-regulation(P＜0.05).Conclusions Recombinant TRAIL protein induces both apoptosis and inflammation of human colon cancer cells.
