中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2010年
3期
187-190
,共4页
杨孝良%巫国辉%李小林%曾瑞
楊孝良%巫國輝%李小林%曾瑞
양효량%무국휘%리소림%증서
瘦素%前脂肪细胞%增殖%分化
瘦素%前脂肪細胞%增殖%分化
수소%전지방세포%증식%분화
Leptin%Preadipocyte%Proliferation%Differentiation
目的 观察瘦索在体外对人前脂肪细胞增殖和分化的影响,探讨瘦素调节肥胖发生的可能机制.方法 分离并体外培养人腹部皮下前脂肪细胞.采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)比色法、细胞计数法、油红O染色提取法及逆转录-聚合酶链式反应(RT-PCR)方法 分析不同浓度(0～1 000 ng/ml)瘦素对人前脂肪细胞增殖、脂质积聚及分化转录因子γ2过氧化物酶体增殖物激活受体(PPAR-γ)、CCAAT增强子α结合蛋白(C/EBP-α)mRNA表达的影响.结果 高浓度(1 000 ng/ml)瘦素能够促进人前脂肪细胞的增殖、脂质积聚以及PPAR-γ2、C/EBP-α mRNA表达(P<0.05).低浓度(10 ng/ml)和中浓度(100 ng/ml)瘦素对人前脂肪细胞的增殖及脂质积聚没有明显的促进作用(P>0.05).结论 在体外超生理浓度的瘦素能够促进前脂肪细胞的增殖和分化,提示瘦素抵抗、血清高瘦素浓度等病理状态时,瘦素可能促进前脂肪细胞的增殖及分化,影响肥胖发生.
目的 觀察瘦索在體外對人前脂肪細胞增殖和分化的影響,探討瘦素調節肥胖髮生的可能機製.方法 分離併體外培養人腹部皮下前脂肪細胞.採用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴鹽(MTT)比色法、細胞計數法、油紅O染色提取法及逆轉錄-聚閤酶鏈式反應(RT-PCR)方法 分析不同濃度(0～1 000 ng/ml)瘦素對人前脂肪細胞增殖、脂質積聚及分化轉錄因子γ2過氧化物酶體增殖物激活受體(PPAR-γ)、CCAAT增彊子α結閤蛋白(C/EBP-α)mRNA錶達的影響.結果 高濃度(1 000 ng/ml)瘦素能夠促進人前脂肪細胞的增殖、脂質積聚以及PPAR-γ2、C/EBP-α mRNA錶達(P<0.05).低濃度(10 ng/ml)和中濃度(100 ng/ml)瘦素對人前脂肪細胞的增殖及脂質積聚沒有明顯的促進作用(P>0.05).結論 在體外超生理濃度的瘦素能夠促進前脂肪細胞的增殖和分化,提示瘦素牴抗、血清高瘦素濃度等病理狀態時,瘦素可能促進前脂肪細胞的增殖及分化,影響肥胖髮生.
목적 관찰수색재체외대인전지방세포증식화분화적영향,탐토수소조절비반발생적가능궤제.방법 분리병체외배양인복부피하전지방세포.채용3-(4,5-이갑기새서-2)-2,5-이분기사담서추염(MTT)비색법、세포계수법、유홍O염색제취법급역전록-취합매련식반응(RT-PCR)방법 분석불동농도(0～1 000 ng/ml)수소대인전지방세포증식、지질적취급분화전록인자γ2과양화물매체증식물격활수체(PPAR-γ)、CCAAT증강자α결합단백(C/EBP-α)mRNA표체적영향.결과 고농도(1 000 ng/ml)수소능구촉진인전지방세포적증식、지질적취이급PPAR-γ2、C/EBP-α mRNA표체(P<0.05).저농도(10 ng/ml)화중농도(100 ng/ml)수소대인전지방세포적증식급지질적취몰유명현적촉진작용(P>0.05).결론 재체외초생리농도적수소능구촉진전지방세포적증식화분화,제시수소저항、혈청고수소농도등병리상태시,수소가능촉진전지방세포적증식급분화,영향비반발생.
Objective To observe the effects of leptin on the proliferation and differentiation of human preadipocyte in vitro, and to explore the possible mechanism of the generation of obesity regulated by leptin. Methods The human preadipocytes were isolated from human subcutaneous adipose tissue of abdomen and cultured in vitro. The effects of leptin (0-1 000 ng/ml) on the proliferation, lipid accumulation and the mRNA expression of PPAR-γ2 and C/EBP-α, which are the differentiation and transcription factor of human preadipocyte, were analyzed by the methods of MTT, cell counting, extracting stained intracytoplasmic lipid with oil red O and RT-PCR. Results Leptin (1 000 ng/ml) could stimulate the proliferation, lipid accumulation and the mRNA expression of PPAR-γ2 and C/EBP-α (P<0. 05). There were not obvious effects on the proliferation and lipid accumulation in the groups of lower (10 ng/ml) and common (100 ng/ml) concentration (P>0. 05). Conclusion Leptin in higher concentration can stimulate the proliferation and differentiation of preadipocye in vitro, which indicates that leptin may regulate the generation of obesity through acting on the proliferation and differentiation of preadipocyte at the pathologic state of leptin resistance and high leptin concentration in serum.
