CAJ | 학술논문

目的:对木犀草素对小鼠干燥性皮炎的作用进行了评价.方法:用丙酮和乙醚的混合液(1∶1)局部敷于ICR小鼠皮肤造成干燥性皮炎模型,造模期间,小鼠皮肤局部应用木犀草素(2,20,100 μg/site),5天后观察45 min内小鼠的自发瘙痒行为,检测造模处皮肤的PGE2含量.此外,我们还对木犀草素体外对compound 48/80(50 μg/ml)引起的大鼠腹腔肥大细胞脱颗粒情况进行了评价.结果:木犀草素能够显著减少干燥性皮炎引起的小鼠的自主瘙痒行为,降低病变皮肤中的PGE2含量,并能有效抑制compound 48/80(50 μg/ml)引起的大鼠腹腔肥大细胞脱颗粒.结论:木犀草素能有效减轻小鼠干燥性皮炎的瘙痒症状,其作用可能是通过对肥大细胞脱颗粒及局部炎症的抑制来实现的.
목적:대목서초소대소서간조성피염적작용진행료평개.방법:용병동화을미적혼합액(1∶1)국부부우ICR소서피부조성간조성피염모형,조모기간,소서피부국부응용목서초소(2,20,100 μg/site),5천후관찰45 min내소서적자발소양행위,검측조모처피부적PGE2함량.차외,아문환대목서초소체외대compound 48/80(50 μg/ml)인기적대서복강비대세포탈과립정황진행료평개.결과:목서초소능구현저감소간조성피염인기적소서적자주소양행위,강저병변피부중적PGE2함량,병능유효억제compound 48/80(50 μg/ml)인기적대서복강비대세포탈과립.결론:목서초소능유효감경소서간조성피염적소양증상,기작용가능시통과대비대세포탈과립급국부염증적억제래실현적.
AIM:To investigate the effects of luteolin on dry skin pruritus in mice.METHOD:Prepare the dry skin prurtius model in ICR mice with cutaneous application of acetone/diethylether(1∶1) and luteolin was locally painted on the treated place(2,20,100 μg/site). Spontaneous scratching times were counted for 45 minutes,and the PGE2 content in the treated place was measured after scratching observation.We still investigated the effects of luteolin on the degranulation of mast cells induced by compound 48/80(50 μg/ml)in vitro.RESULT:Luteolin significantly inhibited the times of scratching behavior associated with dry skin pruritus,decreased the level of PGE2 located in the treated skin and blocked the degranulation of mast cells evoked by compound 48/80 in vitro. Meanwhile,histological examination demonstrated that luteolin can reduce the infiltration of lymphocytes and inflammatory cells and ameliorate the histopathological changes in the dermis.CONCLUTION:Luteolin can reduce the spontaneous scratching behavior associated with dry skin pruritus in mice and its effects against pruritus are maybe contributed to its inhibition on mast cell activation and anti-inflammatory activities.