临床输血与检验
臨床輸血與檢驗
림상수혈여검험
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE
2009年
3期
229-231
,共3页
马曙轩%徐樨巍%宋文琪%薛晖%邵华%安璟云%田京辉
馬曙軒%徐樨巍%宋文琪%薛暉%邵華%安璟雲%田京輝
마서헌%서서외%송문기%설휘%소화%안경운%전경휘
儿童%人类免疫缺陷病毒抗体%人类免疫缺陷综合征
兒童%人類免疫缺陷病毒抗體%人類免疫缺陷綜閤徵
인동%인류면역결함병독항체%인류면역결함종합정
Children Anti-human immunodeficiency virus antibody Aquired immune deficiency syndrome(AIDS)
目的 了解儿科患者手术或输血前抗-HIV初筛与确证实验结果及患病儿童感染HIV的主要途径.方法 对某儿童医院手术或输血前患儿的血清采用酶联免疫吸附试验(ELISA)进行抗-HIV初筛,初筛阳性标本送市疾控中心用免疫印迹法进行确证.分析抗-HIV确证实验阳性患儿的年龄、性别、籍贯及感染原因.结果 共筛查83 329例住院及门诊患儿,初筛阳性患儿47例, 确证实验阳性患儿27例,可疑2例,阴性18例.确证阳性患儿中19例有输血及血液制品史,5例为母婴垂直传播, 3例感染原因不详.50%以上患儿来自河南、山西等艾滋病疫情较重省份.结论 ELISA法检测在儿童抗-HIV初筛中有一定的假阳性率;输血及血制品是医院就诊患儿感染HIV的主要途径.
目的 瞭解兒科患者手術或輸血前抗-HIV初篩與確證實驗結果及患病兒童感染HIV的主要途徑.方法 對某兒童醫院手術或輸血前患兒的血清採用酶聯免疫吸附試驗(ELISA)進行抗-HIV初篩,初篩暘性標本送市疾控中心用免疫印跡法進行確證.分析抗-HIV確證實驗暘性患兒的年齡、性彆、籍貫及感染原因.結果 共篩查83 329例住院及門診患兒,初篩暘性患兒47例, 確證實驗暘性患兒27例,可疑2例,陰性18例.確證暘性患兒中19例有輸血及血液製品史,5例為母嬰垂直傳播, 3例感染原因不詳.50%以上患兒來自河南、山西等艾滋病疫情較重省份.結論 ELISA法檢測在兒童抗-HIV初篩中有一定的假暘性率;輸血及血製品是醫院就診患兒感染HIV的主要途徑.
목적 료해인과환자수술혹수혈전항-HIV초사여학증실험결과급환병인동감염HIV적주요도경.방법 대모인동의원수술혹수혈전환인적혈청채용매련면역흡부시험(ELISA)진행항-HIV초사,초사양성표본송시질공중심용면역인적법진행학증.분석항-HIV학증실험양성환인적년령、성별、적관급감염원인.결과 공사사83 329례주원급문진환인,초사양성환인47례, 학증실험양성환인27례,가의2례,음성18례.학증양성환인중19례유수혈급혈액제품사,5례위모영수직전파, 3례감염원인불상.50%이상환인래자하남、산서등애자병역정교중성빈.결론 ELISA법검측재인동항-HIV초사중유일정적가양성솔;수혈급혈제품시의원취진환인감염HIV적주요도경.
Objective To approach the positive rate of anti-HIV in children receiving pre-operation or pre-transfusion tests,and to find out the primary way of HIV infection in hospitalized children.Methods The samples from the children receiving pre-operation or pre-transfusion tests were detected by enzyme linked immunosorbent assay(ELISA) kit and the positive samples were were taken to the laboratory of CDC in Beijing to confirm. The age, sex, born place and ways of infection in HIV positive children were analyzed.Results Altogether 83 329 children were detected,47 samples were positive in the preliminary tests,of whom 27 children turned positive 2 douwbtful and 18 negative by western blot assay.Of the 27 HIV positive children, 19 ones had the history of blood or blood products transfusion. Five children were transmitted by their mother and the way of infection was unknown in 3 children. More than half of the positive children came from the main prevalence area of acquired immune deficiency syndrome(AIDS) such as Henan and Shanxi province.Conclusion In hospitalized children there was some false positivities in the anti-HIV screening test by ELISA. The primary way of HIV infection in hospitalized children was transfusion of blood or blood products.