CAJ | 학술논문

目的:探讨12-脂氧化酶的活性代谢产物12(S)-HETE对系膜细胞和肾小球内p27~(kip1)表达的影响.方法:12(S)-HETE刺激的系膜细胞和皮下包埋的微型渗透泵长期持续恒速注入12(S)-HETE刺激的大鼠提取的肾小球来观察p27~(kip1)的表达.采用总蛋白量/细胞数的比值作为细胞肥大的评价指标,利用RT-PCR和Western blot方法检测p27~(kip1) mRNA和蛋白的表达.结果:12(S)-HETE刺激能够直接诱导系膜细胞发生肥大.12(S)-HETE刺激的系膜细胞内p27~(kip1)mRNA水平没有变化,而p27~(kip1)蛋白的表达明显增加.然而,在微型渗透泵持续恒速注入12(S)-HETE刺激的大鼠提取的肾小球内p27kip1mRNA水平和蛋白的表达均增加.结论:12(S)-HETE能够通过促进p27~(kip1)的高表达而参与细胞周期的调控,是引起肾小球系膜细胞和肾小球肥大、衰老的重要原因之一.
목적:탐토12-지양화매적활성대사산물12(S)-HETE대계막세포화신소구내p27~(kip1)표체적영향.방법:12(S)-HETE자격적계막세포화피하포매적미형삼투빙장기지속항속주입12(S)-HETE자격적대서제취적신소구래관찰p27~(kip1)적표체.채용총단백량/세포수적비치작위세포비대적평개지표,이용RT-PCR화Western blot방법검측p27~(kip1) mRNA화단백적표체.결과:12(S)-HETE자격능구직접유도계막세포발생비대.12(S)-HETE자격적계막세포내p27~(kip1)mRNA수평몰유변화,이p27~(kip1)단백적표체명현증가.연이,재미형삼투빙지속항속주입12(S)-HETE자격적대서제취적신소구내p27kip1mRNA수평화단백적표체균증가.결론:12(S)-HETE능구통과촉진p27~(kip1)적고표체이삼여세포주기적조공,시인기신소구계막세포화신소구비대、쇠로적중요원인지일.
Objective:To investigate the effect of 12(S)-HETE on the p27~(kip1) expression in mesangial cells and glomeruli.Methods:Mesangial cells were exposed to 12(S)-HETE.12(S)-HETE was infused to rats by osmotic mini-pump.Total protein content measurement for cell hypertrophy,RT-PCR for mRNA expression and Western blot for protein expression were performed respectively.Results:12(S)-HETE stimulation induced mesangial cell hypertrophy and p27~(kip1) protein expression,but not p27~(kip1) mRNA expression.Furthermore,p27~(kip1) mRNA and protein expression in the glomeruli were significantly increased by 12(S)-HETE stimulation using osmotic mini-pump.Conclusion:12(S)-HETE plays an important role in the pathogenesis of glomerular cell hypertrophy and senescence through upregulation of p27~(kip1) expression.