CAJ | 학술논문

用银染法对不同倍性(单倍体、正常二倍体、正常三倍体、雌核发育二倍体、雌核发育鱼子代三倍体)大黄鱼胚胎、仔鱼或成鱼的不同组织进行细胞核仁数目观察计数.结果表明,大黄鱼各种倍性个体细胞核仁数目与染色体倍性之间有良好的对应关系,且核仁数随个体倍性的增加而增加;非诱导雌核发育的大黄鱼细胞核仁众数与染色体组数相等(二倍体为2,三倍体为3),而人工诱导雌核发育大黄鱼及其子代细胞核仁众数却比其相同倍性的非雌核发育鱼少1(雌核发育二倍体为1,雌核发育三倍体为2).雌核发育鱼核仁数减少是一个非常有趣的现象,值得进一步研究.大黄鱼不同发育阶段(胚胎、仔鱼、成鱼)以及同一个体不同组织(鳃、肾、鳍)的细胞核仁数目(众数)相同.因此,借助核仁银染法可以快速、可靠、简便地进行染色体倍性鉴定.而雌核发育鱼核仁数减少的机理值得进一步研究.
용은염법대불동배성(단배체、정상이배체、정상삼배체、자핵발육이배체、자핵발육어자대삼배체)대황어배태、자어혹성어적불동조직진행세포핵인수목관찰계수.결과표명,대황어각충배성개체세포핵인수목여염색체배성지간유량호적대응관계,차핵인수수개체배성적증가이증가;비유도자핵발육적대황어세포핵인음수여염색체조수상등(이배체위2,삼배체위3),이인공유도자핵발육대황어급기자대세포핵인음수각비기상동배성적비자핵발육어소1(자핵발육이배체위1,자핵발육삼배체위2).자핵발육어핵인수감소시일개비상유취적현상,치득진일보연구.대황어불동발육계단(배태、자어、성어)이급동일개체불동조직(새、신、기)적세포핵인수목(음수)상동.인차,차조핵인은염법가이쾌속、가고、간편지진행염색체배성감정.이자핵발육어핵인수감소적궤리치득진일보연구.
Using Ag-staining technique, the nucleoli in interphase cells from different developmental stages (embryo, larva or adult) of large yellow croaker (Pseudosciaena crocea Richardson) were observed and counted, which were in different ploidy levels, including haploid, normal diploid, triploid, gynogenesis diploid and triploid whose mother were gynogenesis diploid. The maximum numbers of nucleoli kept even in the same ploidy samples at different developing stages (embryo, larva or adult), as well as in different tissues(gill, kidney, fin) in any individuals. The maximum numbers of nucleoli of non-gynogenesis fishes (normal diploid and triploid) were 2 and 3 respectively. But it was interesting that the maximum number of the nucleoli from gynogenesis fishes ( gynogenesis diploid with 1 and gynogenesis triploid with 2 ) were one fewer than that of the non-gynogenesis fishes with the same ploidy level, and this phenomona would be deserved deep investigation. The results indicated that the numbers of nucleoli within cells at different ploidy fishes were highly correlated with their ploidy level. Therefore, as a simple and accurate method,silver-staining nucleoli counting could be widely used to identify the ploidy level in fish.