中国老年学杂志
中國老年學雜誌
중국노년학잡지
CHINESE JOURNAL OF GERONTOLOGY
2010年
2期
182-184
,共3页
甘萍%李艾珊%聂桂丽%范英昌
甘萍%李艾珊%聶桂麗%範英昌
감평%리애산%섭계려%범영창
多西环素%乳腺癌%基质金属蛋白酶-2%金属蛋白酶组织抑制因子-2
多西環素%乳腺癌%基質金屬蛋白酶-2%金屬蛋白酶組織抑製因子-2
다서배소%유선암%기질금속단백매-2%금속단백매조직억제인자-2
Doxycycline%Breast cancer%Matrix metalloproteinase (MMP)-2%Tissue inhibitor of metalloproteinase (TIMP)-2
目的 研究多西环素在小鼠乳腺癌肺转移模型中对基质金属蛋白酶-2(MMP-2)及金属蛋白酶组织抑制因子-2(TIMP-2)mRNA表达的影响.方法 30只TAⅡ近交系小鼠建立肺转移性乳腺癌BCML-TAⅡ99模型,随机分为对照组及给药组,每组15只,于可触及肿物后次日,给药组腹腔注射盐酸多西环素0.1 ml(10 mg/ml),对照组给予等量生理盐水.7 w后处死动物,剥离肿瘤称重,部分新鲜组织采用逆转录多聚酶链反应(RT-PCR)方法 检测肿瘤组织MMP-2及TIMP-2 mRNA表达水平.结果 多西环素给药组小鼠肿瘤生长大小及转移率分别为(2.718±0.337)g和13.3%均低于对照组[(4.313±0.468)g,53.3%,P＜0.01,P＜0.05].与对照组比较,多西环素治疗组MMP-2 mRNA为0.637±0.088,明显低于对照组 (1.035±0.097,P＜0.05);TIMP-2 mRNA为0.778±0.148,明显高于对照组 (0.484±0.125,P＜0.05).结论 多西环素通过调节MMP-2及TIMP-2 mRNA的表达水平,对小鼠乳腺癌肺转移模型中肿瘤生长及转移发挥抑制作用.
目的 研究多西環素在小鼠乳腺癌肺轉移模型中對基質金屬蛋白酶-2(MMP-2)及金屬蛋白酶組織抑製因子-2(TIMP-2)mRNA錶達的影響.方法 30隻TAⅡ近交繫小鼠建立肺轉移性乳腺癌BCML-TAⅡ99模型,隨機分為對照組及給藥組,每組15隻,于可觸及腫物後次日,給藥組腹腔註射鹽痠多西環素0.1 ml(10 mg/ml),對照組給予等量生理鹽水.7 w後處死動物,剝離腫瘤稱重,部分新鮮組織採用逆轉錄多聚酶鏈反應(RT-PCR)方法 檢測腫瘤組織MMP-2及TIMP-2 mRNA錶達水平.結果 多西環素給藥組小鼠腫瘤生長大小及轉移率分彆為(2.718±0.337)g和13.3%均低于對照組[(4.313±0.468)g,53.3%,P＜0.01,P＜0.05].與對照組比較,多西環素治療組MMP-2 mRNA為0.637±0.088,明顯低于對照組 (1.035±0.097,P＜0.05);TIMP-2 mRNA為0.778±0.148,明顯高于對照組 (0.484±0.125,P＜0.05).結論 多西環素通過調節MMP-2及TIMP-2 mRNA的錶達水平,對小鼠乳腺癌肺轉移模型中腫瘤生長及轉移髮揮抑製作用.
목적 연구다서배소재소서유선암폐전이모형중대기질금속단백매-2(MMP-2)급금속단백매조직억제인자-2(TIMP-2)mRNA표체적영향.방법 30지TAⅡ근교계소서건립폐전이성유선암BCML-TAⅡ99모형,수궤분위대조조급급약조,매조15지,우가촉급종물후차일,급약조복강주사염산다서배소0.1 ml(10 mg/ml),대조조급여등량생리염수.7 w후처사동물,박리종류칭중,부분신선조직채용역전록다취매련반응(RT-PCR)방법 검측종류조직MMP-2급TIMP-2 mRNA표체수평.결과 다서배소급약조소서종류생장대소급전이솔분별위(2.718±0.337)g화13.3%균저우대조조[(4.313±0.468)g,53.3%,P＜0.01,P＜0.05].여대조조비교,다서배소치료조MMP-2 mRNA위0.637±0.088,명현저우대조조 (1.035±0.097,P＜0.05);TIMP-2 mRNA위0.778±0.148,명현고우대조조 (0.484±0.125,P＜0.05).결론 다서배소통과조절MMP-2급TIMP-2 mRNA적표체수평,대소서유선암폐전이모형중종류생장급전이발휘억제작용.
Objective To study effect and mechanism of doxycycline on the mRNA expressions of matrix metalloproteinase (MMP)-2 and tissue inhibitor of metalloproteinase (TIMP)-2 in breast cancer with lung metastasis model mice (BCML-TAⅡ99). Methods 30 TAⅡ99 mice of breast cancer models with lung metastasis were randomly divided into doxycycline treatment group and control group. Mice in treatment group were injected with doxycycline and those in control group with physiological saline solution everyday when tumor could be touched. 7 weeks later, all mice were killed. All tumors were peeled off and weighted, then small parts of tumors were used to detect the expressions of MMP-2 mRNA and TIMP-2 mRNA by the RT-PCR. Results Both tumor weight and metastasis rate in treatment group were lower than those of control group (P＜0.05). The expression of MMP-2 mRNA was lower and the expression of TIMP-2 mRNA was higher in treatment group than that of control group (P＜0.05). Conclusions Doxycycline can inhibit tumor growth and metastasis in breast cancer with lung metastasis model mice by regulating MMP-2 mRNA and TIMP-2 mRNA expression.
