CAJ | 학술논문

目的:探讨O6-甲基鸟嘌呤-DNA甲基转移酶(O6-methylguanine-DNA methyltransferase, MGMT)在甲状腺癌组织中的表达及其临床意义.方法:用免疫组织化学SP法检测61例甲状腺癌、21例甲状腺良性腺瘤、15例桥本氏甲状腺炎、8例结节性甲状腺肿和12例癌旁正常组织中MGMT蛋白的表达,并结合临床病理因素进行分析.结果:MGMT在甲状腺癌组织中的表达与正常组织中的表达差异有统计学意义(P＜0.05).MGMT表达从正常组织(16.67%,10/12)、结节性甲状腺肿(25.00%,2/8)、桥本氏甲状腺炎(60.00%,9/15)、甲状腺腺瘤(52.38%,11/21)到甲状腺癌(60.66%,38/61)中表达水平基本呈上升趋势.在甲状腺乳头状癌与甲状腺滤泡癌的表达差异有统计学意义(P＜0.05),表达水平随甲状腺癌恶性程度的增加而降低,分别为乳头状癌(72.22%,26/36)、滤泡癌(50.00%,8/16).MGMT在性别、年龄及民族组中表达差异均无统计学意义(P＞0.05).结论:MGMT高表达现象可能与甲状腺癌恶性程度有关,可成为候选的临床分子诊断指标.甲状腺癌组织的MGMT蛋白在性别、年龄和民族组表达均无差异,有望成为通用的临床检测指标.
목적:탐토O6-갑기조표령-DNA갑기전이매(O6-methylguanine-DNA methyltransferase, MGMT)재갑상선암조직중적표체급기림상의의.방법:용면역조직화학SP법검측61례갑상선암、21례갑상선량성선류、15례교본씨갑상선염、8례결절성갑상선종화12례암방정상조직중MGMT단백적표체,병결합림상병리인소진행분석.결과:MGMT재갑상선암조직중적표체여정상조직중적표체차이유통계학의의(P＜0.05).MGMT표체종정상조직(16.67%,10/12)、결절성갑상선종(25.00%,2/8)、교본씨갑상선염(60.00%,9/15)、갑상선선류(52.38%,11/21)도갑상선암(60.66%,38/61)중표체수평기본정상승추세.재갑상선유두상암여갑상선려포암적표체차이유통계학의의(P＜0.05),표체수평수갑상선암악성정도적증가이강저,분별위유두상암(72.22%,26/36)、려포암(50.00%,8/16).MGMT재성별、년령급민족조중표체차이균무통계학의의(P＞0.05).결론:MGMT고표체현상가능여갑상선암악성정도유관,가성위후선적림상분자진단지표.갑상선암조직적MGMT단백재성별、년령화민족조표체균무차이,유망성위통용적림상검측지표.
Objective To study the expression of O6-methylguanine-DNA methyltransferase (MGMT) and its clinicopathological significance in thyroid cancer.Methods Immunohistochemistry was used to determine the expression of MGMT in 61 thyroid cancer tissues, 21 thyroid adenomas, 15 Hashimoto's thyroiditis, 8 nodular goiter, and 12 peri-tumor tissues. Results There was statistic difference in the expression of MGMT between the normal tissues and thyroid cancers (P＜0.05). Expression of MGMT increased from the normal tissue (16.67%, 10/12), nodular goiter (25.00%, 2/8), Hashimoto's thyroiditis (60.00%, 9/15), and thyroid adenoma (52.38%, 11/21)to thyroid cancer (60.66%, 38/61). Expression of MGMT in papillary thyroid cancer (PTC) and follicular thyroid cancer (FTC) had significant difference (P＜0.05), and the expression level of MGMT decreased with the malignancy of thyroid cancer, such as in PTC (72.22%, 26/36), and FTC (50.00%, 8/16). There was no statistic difference in MGMT expression in sex, age, and nationality (P＞0.05).Conclusion High expression of MGMT might be related to the malignancy of thyroid cancer, which may be one of the diagnosis indexes for thyroid cancer. It will be a common clinical index in diagnosingthyroid cancer since there is no difference in MGMT expression among sexes, ages, and nationalities.