CAJ | 학술논문

目的利用血管内超声技术观察脂联素对动脉粥样硬化斑块的影响.方法构建携带脂联素基因的腺病毒表达载体(Ad-APN),将其转染到已形成粥样硬化斑块的新西兰大白兔腹主动脉内膜,通过血管内超声和病理学测量斑块的面积.结果新西兰大白兔腹主动脉局部转染Ad-APN后,斑块面积与对照组相比缩小36.39%(P＜0.01),管腔面积狭窄率减小23.37%(P＜0.05);Ad-APN组与转染前比较斑块面积缩小37.50%(P＜0.01),管腔面积狭窄率减小33.15%(P＜0.01).油红O染色结果相似,局部转染Ad-APN使斑块面积缩小30.70%(P＜0.05),斑块最大厚度减小20.83%(P＜0.05).结论血管内超声技术能在体准确评价脂联素对动脉粥样硬化斑块的作用.
목적 이용혈관내초성기술관찰지련소대동맥죽양경화반괴적영향.방법 구건휴대지련소기인적선병독표체재체(Ad-APN),장기전염도이형성죽양경화반괴적신서란대백토복주동맥내막,통과혈관내초성화병이학측량반괴적면적.결과 신서란대백토복주동맥국부전염Ad-APN후,반괴면적여대조조상비축소36.39%(P＜0.01),관강면적협착솔감소23.37%(P＜0.05);Ad-APN조여전염전비교반괴면적축소37.50%(P＜0.01),관강면적협착솔감소33.15%(P＜0.01).유홍O염색결과상사,국부전염Ad-APN사반괴면적축소30.70%(P＜0.05),반괴최대후도감소20.83%(P＜0.05).결론 혈관내초성기술능재체준학평개지련소대동맥죽양경화반괴적작용.
Objective To investigate the effects of adiponectin on the development of atherosclerosis.Methods The in vivo role of adiponectin on the development of atherosclerosis in rabbits was investigated mainly using adiponectin-producing adenovirus(Ad-APN)and intravascular ultrasound(IVUS).On day 14 after Ad-APN local transfer to the intima of abdominal aortas,abdominal IVUS images were recorded and then rabbits were sacrificed.Abdominal aortas were collected and performed histochemical analysis.Results Ultrasonography revealed a significantly reduced atherosclerotic area,in abdominal aortas of rabbits infected through intima with Ad-APN,by 36.39% compared with the area in adenovirus expressing β galactosidase gene(Ad-βgal)treated rabbits(P＜0.01),and by 37.50% compared with that before treatment(P＜0.01).The lumen area stenosis was also reduced by 23.37%(P＜0.05)and 33.15%(P＜0.01),respectively.In rabbits with Ad-APN infection,the atherosclerotic plaque area as seen on Oil Red O staining was reduced significantly,by 30.70%(P＜0.05)and the greatest thickness of plaque was reduced,by 20.83%(P＜0.05)as compared with those in Ad-βgal-treated rabbits,respectively.Conclusions Intravascular ultrasound analysis is a valuable method in the in vivo study about the effects of adiponectin on the development of atherosclerosis.