CAJ | 학술논문

目的调查多重耐药大肠埃希菌耐药相关基因与可移动遗传元件的相互关系,以及菌株间的亲缘性关系.方法收集浙江省磐安县人民医院2009年6月-2010年6月临床分离的多重耐药大肠埃希菌共20株,采用聚合酶链反应(PCR)分析β-内酰胺类与氨基糖苷类耐药相关基因以及可移动遗传元件,并对检测结果作指标与样本聚类分析.结果 20株多重耐药大肠埃希菌共检出4种β-内酰胺类耐药相关基因,4种氨基糖苷类耐药相关基因,以及5种可移动遗传元件的遗传标记.指标聚类分析显示,耐药基因TEM、CTX-M-1、aadA5与可移动遗传元件traA、IS26、ISEcpl相关联,耐药基因OXA-1、aac(6′)-Ⅰb、ant(3″)-Ⅰ、rmtB与可移动遗传元件trbC、IS903相关联.样本聚类分析显示,本院20株耐药大肠埃希菌可分为两个群,并且两群菌株演化明显.结论多重耐药大肠埃希菌耐药相关基因与可移动遗传元件有关,对菌株进行样本聚类分析可获得菌株亲缘性关系,对控制医院感染意义重大.
목적 조사다중내약대장애희균내약상관기인여가이동유전원건적상호관계,이급균주간적친연성관계.방법 수집절강성반안현인민의원2009년6월-2010년6월림상분리적다중내약대장애희균공20주,채용취합매련반응(PCR)분석β-내선알류여안기당감류내약상관기인이급가이동유전원건,병대검측결과작지표여양본취류분석.결과 20주다중내약대장애희균공검출4충β-내선알류내약상관기인,4충안기당감류내약상관기인,이급5충가이동유전원건적유전표기.지표취류분석현시,내약기인TEM、CTX-M-1、aadA5여가이동유전원건traA、IS26、ISEcpl상관련,내약기인OXA-1、aac(6′)-Ⅰb、ant(3″)-Ⅰ、rmtB여가이동유전원건trbC、IS903상관련.양본취류분석현시,본원20주내약대장애희균가분위량개군,병차량군균주연화명현.결론 다중내약대장애희균내약상관기인여가이동유전원건유관,대균주진행양본취류분석가획득균주친연성관계,대공제의원감염의의중대.
Objective To investigate the correlation between drug-resistant genes and mobile genetic elements in multi-drug resistant Escherichia coli,and to explore phylogeny among the strains.Methods Totally 20 strains of multi-drug resistant Escherichia coli were collected from Pan' an Hospital,Zhejiang Province during June 2009 and June 2010.Beta-lactam-resistance genes,aminoglycoside-resistance genes,genetic markers of mobile genetic elements were analyzed by PCR.Index and sample cluster analysis were performed on above results. Results In 20 strains of Escherichia coli,4 kinds of beta-lactamresistance genes,4 kinds of aminoglycoside-resistance genes,and 5 kinds of genetic markers of mobile genetic elements were detected.Index cluster analysis showed that correlation existed between resistance genes TEM,CTX-M-1,aadA5 and mobile genetic elements traA,IS26,ISEcpl; and correlation also existed between resistance genes OXA-1,aac(6′)-Ⅰ b,ant(3")-Ⅰ,rmtB and mobile genetic elements trbC,IS903.Sample cluster analysis showed that this group of Escherichia coli could be divided into 2 groups which were genetically different.Conclusions Drug-resistant genes in multi-drug resistant Escherichia coli are correlated with mobile genetic elements.Sample cluster analysis can reveal phylogeny among the strains,which is important for hospital infection control.