中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2008年
6期
404-406
,共3页
宋少伟%刘永锋%梁健%何三光
宋少偉%劉永鋒%樑健%何三光
송소위%류영봉%량건%하삼광
再灌注损伤%嘌呤核苷磷酸酶%透明质酸%大鼠肝脏
再灌註損傷%嘌呤覈苷燐痠酶%透明質痠%大鼠肝髒
재관주손상%표령핵감린산매%투명질산%대서간장
Ischemia reperfusion injury%Purine nucleoside phosphorylase%Hyaluric acid%Rat 1iver
目的 探讨大鼠肝脏低温保存及常温缺血再灌注过程中在不同的保存液中嘌呤核苷磷酸酶(PNP)活性和透明质酸(HA)吸收率的变化.方法 将大鼠肝脏在三种不同保存液中低温保存16 h和24 h后,用37℃Krebs-Henseleit液连续循环灌注90 min,分别于不同灌注时间检测灌洗液中PNP活性和外源性透明质酸的吸收率的变化.结果 经过16 h的低温保存后,再灌注60 min前,HTK保存的肝脏中PNP明显高于uw和Celsior;60 min后HTK和Celsior保存的肝脏中PNP明显高于UW;经过24 h的低温保存后,再灌注15 min后,HTK保存的肝脏中PNP明显高于Celsior,而Celsior又明显高于UW.低温保存16 h后,再灌注时,3种保存液保存的肝脏对外源性透明质酸的吸收率均为负值,表明肝窦内皮细胞受到一定程度的损伤;保存24 h者,UW液保存肝脏外源性透明质酸的吸收率明显高于Celsior液和HTK液.结论 随着低温保存和再灌注时间的延长,大鼠肝脏中PNP活性逐渐增高,而外源性透明质酸的吸收率下降;二者可作为评价肝脏缺血再灌注损伤的指标.
目的 探討大鼠肝髒低溫保存及常溫缺血再灌註過程中在不同的保存液中嘌呤覈苷燐痠酶(PNP)活性和透明質痠(HA)吸收率的變化.方法 將大鼠肝髒在三種不同保存液中低溫保存16 h和24 h後,用37℃Krebs-Henseleit液連續循環灌註90 min,分彆于不同灌註時間檢測灌洗液中PNP活性和外源性透明質痠的吸收率的變化.結果 經過16 h的低溫保存後,再灌註60 min前,HTK保存的肝髒中PNP明顯高于uw和Celsior;60 min後HTK和Celsior保存的肝髒中PNP明顯高于UW;經過24 h的低溫保存後,再灌註15 min後,HTK保存的肝髒中PNP明顯高于Celsior,而Celsior又明顯高于UW.低溫保存16 h後,再灌註時,3種保存液保存的肝髒對外源性透明質痠的吸收率均為負值,錶明肝竇內皮細胞受到一定程度的損傷;保存24 h者,UW液保存肝髒外源性透明質痠的吸收率明顯高于Celsior液和HTK液.結論 隨著低溫保存和再灌註時間的延長,大鼠肝髒中PNP活性逐漸增高,而外源性透明質痠的吸收率下降;二者可作為評價肝髒缺血再灌註損傷的指標.
목적 탐토대서간장저온보존급상온결혈재관주과정중재불동적보존액중표령핵감린산매(PNP)활성화투명질산(HA)흡수솔적변화.방법 장대서간장재삼충불동보존액중저온보존16 h화24 h후,용37℃Krebs-Henseleit액련속순배관주90 min,분별우불동관주시간검측관세액중PNP활성화외원성투명질산적흡수솔적변화.결과 경과16 h적저온보존후,재관주60 min전,HTK보존적간장중PNP명현고우uw화Celsior;60 min후HTK화Celsior보존적간장중PNP명현고우UW;경과24 h적저온보존후,재관주15 min후,HTK보존적간장중PNP명현고우Celsior,이Celsior우명현고우UW.저온보존16 h후,재관주시,3충보존액보존적간장대외원성투명질산적흡수솔균위부치,표명간두내피세포수도일정정도적손상;보존24 h자,UW액보존간장외원성투명질산적흡수솔명현고우Celsior액화HTK액.결론 수착저온보존화재관주시간적연장,대서간장중PNP활성축점증고,이외원성투명질산적흡수솔하강;이자가작위평개간장결혈재관주손상적지표.
Objective To investigate the changes in purine nucleoside phosphorylase(PNP)and exogenous hyaluronic acid(HA)absorption after cold storage and normothermic reperfusion of rat liver in University of Wisconsin(UW),Celsior and histidine-tryptopan-ketoglutarate(HTK)solutions during ischemia and reperfusion. Methods After different time of cold storage in different preserving solutions,the rat liver was reperfused for 90 min with 37℃ Krebs-Henseleit solution using isolated rat liver perfusion model. The PNP and absorption of exogenous HA was determined in perfusate at different time phases of reperfusion. Results After 16 h and before 60-min reperfusion,PNP level of rat liver in HTK was significantly higher than that in UW and Celsior(P<O.O 1).After 60 min, PNP in HTK and Celsior was higher than that in UW. During reperfusion, the rat liver preserved in UW and Celsior had a higher absorption of exogenous HA than that in HTK(P<O.01).Twenty-four hours after cold storage, PNP level in HTK was markedly higher than that in Celsior and the PNP in Celsior was higher than that in UW 15 min after reperfusion. Rat liver preserved in UW had a higher absorption of exogenous HA than that in Celsior and HTK(P<O.01).Conclusion The PNP levelin rat liver is increased and the absorption of exogenous HA is decreased by the prolongation of cold storage and reperfusion.Therefore,both can be used as the indicators of hepatic ischemia/repeal"usion injury.
