国际遗传学杂志
國際遺傳學雜誌
국제유전학잡지
INTERNATIONAL JOURNAL OF GENETICS
2010年
3期
142-146
,共5页
隋钰%卢永平%李学付%冯文华%赵宁%黄芳%胥威%赵淼%韩维田
隋鈺%盧永平%李學付%馮文華%趙寧%黃芳%胥威%趙淼%韓維田
수옥%로영평%리학부%풍문화%조저%황방%서위%조묘%한유전
小扩增子基因分型%高分辨率熔解曲线%SNPs
小擴增子基因分型%高分辨率鎔解麯線%SNPs
소확증자기인분형%고분변솔용해곡선%SNPs
Small amplicon genotyping%High resolution melting%SNPs
目的 应用小扩增子基因分型方法检测正常人宫颈癌易感基因XRCC1 Arg194Trp位点多态性改变.方法 提取10例健康人外周血基因组DNA;PCR扩增XRCC1 基因 Arg194Trp位点,扩增产物进行小扩增子基因分型分析.并应用测序方法对分析结果进行验证.结果 HRM检测结果分别显示3种基因型:XRCC1 Arg194Arg(C/C)、XRCC1 Arg194Trp(C/T)和XRCC1 Trp194Trp(T/T).测序结果与小扩增子基因分型方法分析结果完全一致.结论 应用小扩增子基因分型方法可以对XRCC1 Arg194Trp进行准确分型.
目的 應用小擴增子基因分型方法檢測正常人宮頸癌易感基因XRCC1 Arg194Trp位點多態性改變.方法 提取10例健康人外週血基因組DNA;PCR擴增XRCC1 基因 Arg194Trp位點,擴增產物進行小擴增子基因分型分析.併應用測序方法對分析結果進行驗證.結果 HRM檢測結果分彆顯示3種基因型:XRCC1 Arg194Arg(C/C)、XRCC1 Arg194Trp(C/T)和XRCC1 Trp194Trp(T/T).測序結果與小擴增子基因分型方法分析結果完全一緻.結論 應用小擴增子基因分型方法可以對XRCC1 Arg194Trp進行準確分型.
목적 응용소확증자기인분형방법검측정상인궁경암역감기인XRCC1 Arg194Trp위점다태성개변.방법 제취10례건강인외주혈기인조DNA;PCR확증XRCC1 기인 Arg194Trp위점,확증산물진행소확증자기인분형분석.병응용측서방법대분석결과진행험증.결과 HRM검측결과분별현시3충기인형:XRCC1 Arg194Arg(C/C)、XRCC1 Arg194Trp(C/T)화XRCC1 Trp194Trp(T/T).측서결과여소확증자기인분형방법분석결과완전일치.결론 응용소확증자기인분형방법가이대XRCC1 Arg194Trp진행준학분형.
Objective To detect the SNP polymorphisms of cervical cancer-associated-gene-XRCC1 Arg194Trp using small amplicon genotyping method. Method Genomic DNA was extracted from peripheral blood sample obtained from 10 healthy donors. DNA fragments encompassing XRCC1 Arg194Trp SNP polymorphisms were produced by PCR amplification and analyzed by small amplicon genotyping method. SNP polymorphisms were verified by DNA sequencing. Results showed that there are three genotypes: XRCC1 Arg194Arg (C/C) , XRCC1 Arg194Trp(C/T) , XRCC1 Trp194Trp(T/T). All of the polymorphic results are consistent with those obtained via DNA sequencing. Conclusion Small amplicon genotyping is an accurate and reliable method to detect the XRCC1 Arg194Trp polymorphisms.
