中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2009年
2期
88-91
,共4页
刘雪华%杨瑞年%刘福佳%郭昆华%赵宏颖%高春锦
劉雪華%楊瑞年%劉福佳%郭昆華%趙宏穎%高春錦
류설화%양서년%류복가%곽곤화%조굉영%고춘금
高压氧%皮瓣%血管内皮生长因子%缝隙连接蛋白
高壓氧%皮瓣%血管內皮生長因子%縫隙連接蛋白
고압양%피판%혈관내피생장인자%봉극련접단백
Hyperbaric oxygen%Skin flaps%Vascular endothelial growth factor (VEGF)%Connexin 43(CX43)
目的 探讨高压氧对家兔随意皮瓣毛细血管增殖作用的机制.方法 建立家兔背部随意皮瓣模型,予高压氧治疗,观察第3天和第7天时对照组和高压氧组皮瓣中毛细血管密度及血管内皮细胞生长因子(VEGF)、缝隙连接蛋白(CX43)的含量变化情况.结果 高乐氧组皮瓣毛细血管密度(第3天11.63±2.94,第7天13.84±3.49)明显高于对照组(第3天9.13±2.00.第7天10.51±3.01)(第3天时P<0.05,第7天时P<0.01);第3天和第7天时高压氧组皮瓣VEGF(第3天5.50±1.68,第7天5.81±1.64)表达明显高于对照组(第3天4.55±1.06,第7天4.63±1.24)(分别为P<0.01);高压氧组皮瓣CX43(第3天4.08±0.88,第7天4.60±0.97)表达明显高于对照组(第3天3.28±0.68,第7天3.60±1.01)(第3天时P<0.01,第7天时P<0.05).结论 高压氧治疗促进家兔随意皮瓣毛细血管增殖的作用机制之一可能是通过增加皮瓣组织中VEGF、CX43的表达而促进新生毛细血管形成.
目的 探討高壓氧對傢兔隨意皮瓣毛細血管增殖作用的機製.方法 建立傢兔揹部隨意皮瓣模型,予高壓氧治療,觀察第3天和第7天時對照組和高壓氧組皮瓣中毛細血管密度及血管內皮細胞生長因子(VEGF)、縫隙連接蛋白(CX43)的含量變化情況.結果 高樂氧組皮瓣毛細血管密度(第3天11.63±2.94,第7天13.84±3.49)明顯高于對照組(第3天9.13±2.00.第7天10.51±3.01)(第3天時P<0.05,第7天時P<0.01);第3天和第7天時高壓氧組皮瓣VEGF(第3天5.50±1.68,第7天5.81±1.64)錶達明顯高于對照組(第3天4.55±1.06,第7天4.63±1.24)(分彆為P<0.01);高壓氧組皮瓣CX43(第3天4.08±0.88,第7天4.60±0.97)錶達明顯高于對照組(第3天3.28±0.68,第7天3.60±1.01)(第3天時P<0.01,第7天時P<0.05).結論 高壓氧治療促進傢兔隨意皮瓣毛細血管增殖的作用機製之一可能是通過增加皮瓣組織中VEGF、CX43的錶達而促進新生毛細血管形成.
목적 탐토고압양대가토수의피판모세혈관증식작용적궤제.방법 건립가토배부수의피판모형,여고압양치료,관찰제3천화제7천시대조조화고압양조피판중모세혈관밀도급혈관내피세포생장인자(VEGF)、봉극련접단백(CX43)적함량변화정황.결과 고악양조피판모세혈관밀도(제3천11.63±2.94,제7천13.84±3.49)명현고우대조조(제3천9.13±2.00.제7천10.51±3.01)(제3천시P<0.05,제7천시P<0.01);제3천화제7천시고압양조피판VEGF(제3천5.50±1.68,제7천5.81±1.64)표체명현고우대조조(제3천4.55±1.06,제7천4.63±1.24)(분별위P<0.01);고압양조피판CX43(제3천4.08±0.88,제7천4.60±0.97)표체명현고우대조조(제3천3.28±0.68,제7천3.60±1.01)(제3천시P<0.01,제7천시P<0.05).결론 고압양치료촉진가토수의피판모세혈관증식적작용궤제지일가능시통과증가피판조직중VEGF、CX43적표체이촉진신생모세혈관형성.
Objective To explore effects and mechanism of hyperbaric oxygen (HBO) on capillary proliferation of random skin flaps in rabbits. Methods Random skin flap models were developed on the back of rabbits and HBO was administered to rabbits in the HBO group. Capillary density and contents of VEGF, CX43 were observed on the 3rd day and the 7th day respectively. Results Capillary density of the animals in the HBO group were higher than those in the control group on the 3rd day (P<0.05) and the 7th day (P<0.01) respectively. The content of VEGF in the HBO group was higher than that in the control group on the 3rd day and the 7th day (P<0.05). The contents of CX43 in the HBO group were higher than that in the control group on 3rd day (P<0.01) and the 7th day (P<0.05) respectively. Conclusions One of the mechanisms of HBO might be that higher expression of VEGF and CX43 promoted capillary proliferation in random skin flaps.
