中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2010年
6期
530-534
,共5页
茶多酚%HL-60细胞%细胞凋亡%细胞周期%体外研究
茶多酚%HL-60細胞%細胞凋亡%細胞週期%體外研究
다다분%HL-60세포%세포조망%세포주기%체외연구
Tea polyphenols (TP)%HL-60 cells%Apoptosis%Cell cycle%In vitro study
目的 探讨茶多酚对人急性早幼粒白血病细胞株HL-60细胞增殖和细胞周期的影响.方法 采用四甲基偶氮唑盐微量酶反应比色法(MTT比色法)观察茶多酚对体外培养的HL-60细胞增殖活性的影响.采用HE染色、荧光染色观察用茶多酚后细胞形态变化.用流式细胞仪(FACS)检测细胞凋亡率及细胞周期.结果 (1)MTT比色法检测显示茶多酚能抑制HL-60细胞增殖,在一定范围内呈剂量和时间依赖性(P＜0.05).当茶多酚浓度达到400和800 mg/L时,48 h抑制率分别为(58.90±1.19)%和(72.57±0.70)%.(2)流式细胞仪分析,茶多酚处理组出现一特征性的亚二倍体凋亡峰.其凋亡率呈时间、剂量依赖性.(3)流式细胞术发现茶多酚可使HL-60细胞阻滞于S期,其阻滞细胞的数量与药物浓度呈正相剂量关系,以作用24 h对细胞周期的阻滞作用最强.结论 茶多酚能有效抑制HL-60细胞增殖,在一定范围内具有时间和剂量依赖性.茶多酚可体外诱导HL-60细胞凋亡,并使细胞周期阻滞于S期.
目的 探討茶多酚對人急性早幼粒白血病細胞株HL-60細胞增殖和細胞週期的影響.方法 採用四甲基偶氮唑鹽微量酶反應比色法(MTT比色法)觀察茶多酚對體外培養的HL-60細胞增殖活性的影響.採用HE染色、熒光染色觀察用茶多酚後細胞形態變化.用流式細胞儀(FACS)檢測細胞凋亡率及細胞週期.結果 (1)MTT比色法檢測顯示茶多酚能抑製HL-60細胞增殖,在一定範圍內呈劑量和時間依賴性(P＜0.05).噹茶多酚濃度達到400和800 mg/L時,48 h抑製率分彆為(58.90±1.19)%和(72.57±0.70)%.(2)流式細胞儀分析,茶多酚處理組齣現一特徵性的亞二倍體凋亡峰.其凋亡率呈時間、劑量依賴性.(3)流式細胞術髮現茶多酚可使HL-60細胞阻滯于S期,其阻滯細胞的數量與藥物濃度呈正相劑量關繫,以作用24 h對細胞週期的阻滯作用最彊.結論 茶多酚能有效抑製HL-60細胞增殖,在一定範圍內具有時間和劑量依賴性.茶多酚可體外誘導HL-60細胞凋亡,併使細胞週期阻滯于S期.
목적 탐토다다분대인급성조유립백혈병세포주HL-60세포증식화세포주기적영향.방법 채용사갑기우담서염미량매반응비색법(MTT비색법)관찰다다분대체외배양적HL-60세포증식활성적영향.채용HE염색、형광염색관찰용다다분후세포형태변화.용류식세포의(FACS)검측세포조망솔급세포주기.결과 (1)MTT비색법검측현시다다분능억제HL-60세포증식,재일정범위내정제량화시간의뢰성(P＜0.05).당다다분농도체도400화800 mg/L시,48 h억제솔분별위(58.90±1.19)%화(72.57±0.70)%.(2)류식세포의분석,다다분처리조출현일특정성적아이배체조망봉.기조망솔정시간、제량의뢰성.(3)류식세포술발현다다분가사HL-60세포조체우S기,기조체세포적수량여약물농도정정상제량관계,이작용24 h대세포주기적조체작용최강.결론 다다분능유효억제HL-60세포증식,재일정범위내구유시간화제량의뢰성.다다분가체외유도HL-60세포조망,병사세포주기조체우S기.
Objective To investigate the in vitro effects of tea polyphenols (TP) on proliferation and cell cycle of human acute promyelocytic leukemia cell line HL-60. Methods The in vitro effects of TP on proliferation of HL-60 cells were analyzed by MTT colorimetric assay. The morphologic changes of HL-60 cells after TP treatment were observed by HE staining and immunofluorescence staining, and flow cytometry was used to examine the cell apoptosis rate and cell cycle. Results ①The result of MTT colormetric assay revealed that TP inhibited the proliferation of HL-60 cells in dose- and time-dependent manner within a certain range of dosage (P＜0.05). With 400 mg/L or 800 mg/L TP, the 48 h inhibition rate of the HL-60 cells was (58.90± 1.19) ％ and (72.57±0.70) ％, respectively. ②By using flow cytometry, a distinctive hypodiploid peak was observed in HL-60 cells cultured with TP, and the apoptosis rate was dose- and time-dependent.(3) HL-60 cells were arrested at S phase and the number of arrested cells was positively correlated with drug dose. The strongest blockage effect of TP was observed at 24 h. Conclusions Within a certain range of dosage, TP can inhibit the proliferation of HL-60 cells in dose- and time-dependent manner. TP can induce HL-60 cells apoptosis and block the cell cycle of HL-60 cells at S phase in vitro.
