CAJ | 학술논문

目的观察阿奇霉素对哮喘小鼠气道重构指标、转化生长因子-β1(transforming growth factor-β1,TGF-β1)表达的影响,探讨阿奇霉素对哮喘小鼠气道重构的影响.方法 30只雄性BALB/c小鼠随机分为正常对照组(A组)、哮喘模型组(B组)、阿奇霉素治疗组(C组),每组各10只,复制哮喘模型后收集支气管肺泡灌洗液(BALF),行白细胞(TOT),嗜酸性粒细胞(EOS)计数;医学图像分析软件观测支气管总管壁面积(Wat)、内壁面积(Wai)、平滑肌面积(Wam);SABC免疫组化法检测肺组织TGF-β1的表达.结果 B组BALF中嗜酸性粒细胞明显高于其他组(8.12±0.54 vs 0.70±0.40,8.12±0.54 vs 0.87±0.25,P＜0.01);C组气道Wat、Wai、Wam均较B组减轻(10.15±0.95 vs 15.36±0.85,4.16±0.32 vs 10.64±1.03,3.77±0.15 vs 7.97±0.17,P＜0.01),但较A组加重;B组TGF-β1(168.58±21.71 vs 130.47±18.22 vs 126.30±16.15)表达最强,C组减弱,A组微弱;TGF-β1的表达与EOS%(r=0.840,P＜0.01)、Wat(r=0.735,P＜0.01)、Wam(r=0.870,P＜0.01)呈正相关.结论阿奇霉素可抑制哮喘小鼠气道重构,可能是通过抑制TGF-β1的表达来实现的.
목적 관찰아기매소대효천소서기도중구지표、전화생장인자-β1(transforming growth factor-β1,TGF-β1)표체적영향,탐토아기매소대효천소서기도중구적영향.방법 30지웅성BALB/c소서수궤분위정상대조조(A조)、효천모형조(B조)、아기매소치료조(C조),매조각10지,복제효천모형후수집지기관폐포관세액(BALF),행백세포(TOT),기산성립세포(EOS)계수;의학도상분석연건관측지기관총관벽면적(Wat)、내벽면적(Wai)、평활기면적(Wam);SABC면역조화법검측폐조직TGF-β1적표체.결과 B조BALF중기산성립세포명현고우기타조(8.12±0.54 vs 0.70±0.40,8.12±0.54 vs 0.87±0.25,P＜0.01);C조기도Wat、Wai、Wam균교B조감경(10.15±0.95 vs 15.36±0.85,4.16±0.32 vs 10.64±1.03,3.77±0.15 vs 7.97±0.17,P＜0.01),단교A조가중;B조TGF-β1(168.58±21.71 vs 130.47±18.22 vs 126.30±16.15)표체최강,C조감약,A조미약;TGF-β1적표체여EOS%(r=0.840,P＜0.01)、Wat(r=0.735,P＜0.01)、Wam(r=0.870,P＜0.01)정정상관.결론 아기매소가억제효천소서기도중구,가능시통과억제TGF-β1적표체래실현적.
Objective To investigate the effects of azithromycin on airway remodeling and the expression of transforming growth factorβ1 (TGF-β1) in asthmatic mice.Methods BALB/c male mice were random divided into 3 groups:control group(A),asthma group (B),and azithromycin treated group (C),with 10 mice in each group.Bronchoalveolar lavage fluid was collected to count the number of white blood cells and eosinophilic granulocytes EOS.The morphological parameters of the bronchi were measured by computer image analysis and the pathologic changes of the bronchi and lung tissue were observed by HE staining.The expressions of TGF-β1 were detected by immunohistochemistry.Results The number of EOS in B group was significantly higher than that in control group(8.12 ±0.54 vs 0.70 ±0.40;8.12 ±0.54 vs 0.87 ±0.25,P ＜0.01).WAt and WAi and WAm in group C was significantly lower than that in group B (10.15 ±0.95 vs 15.36 ±0.85,4.16 ±0.32 vs 10.64 ± 1.03,3.77 ±0.15 vs 7.97 ±0.17,P ＜0.01)but higher than that in group A.The expression of TGF-β1 in group C was significantly lower than that in group B but higher than that in group A.TGF-β1 expression of lung tissue in C group was significantly correlated with EOS,(r =0.840,P ＜0.01) and WAt(r =0.735,P ＜0.01) and WAm (r =0.870,P ＜0.01).Conclusion Azithromycin inhibited airway remodeling in asthmatic mice,which might possibly be achieved through inhibiting the expression of TGF-β1.