中国危重病急救医学
中國危重病急救醫學
중국위중병급구의학
CHINESE CRITICAL CARE MEDICINE
2010年
5期
282-284,后插1
,共4页
廖秀玉%林建东%倪秀雄%林辉
廖秀玉%林建東%倪秀雄%林輝
료수옥%림건동%예수웅%림휘
还原型谷胱甘肽%脓毒症%细胞因子%肿瘤坏死因子-α%白细胞介素-6%超微结构
還原型穀胱甘肽%膿毒癥%細胞因子%腫瘤壞死因子-α%白細胞介素-6%超微結構
환원형곡광감태%농독증%세포인자%종류배사인자-α%백세포개소-6%초미결구
Reduced glutathione%Sepsis%Cytokine%Tumor necrosis factor-α%Interleukin-6 Uhrastructure
目的 探讨还原型谷胱甘肽(GSH)对脓毒症大鼠急性肺损伤保护作用的机制.方法 采用盲肠结扎穿孔术(CLP)复制SD大鼠脓毒症模型.按随机数字表法将大鼠分为假手术组、模型组、GSH组、左氧氟沙星(LEV)组;分别于术后3、6、12、24 h各取7只大鼠心脏血检测血浆肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平,电镜下观察术后24 h大鼠肺组织超微结构的改变.结果 与假手术组C(132±9)μg/L]比较,模型组术后6 h血浆TNF-α水平((227±28)μg/L]显著升高(zp<0.01);GSH治疗组((144±28)μg/L]较模型组显著降低,且明显低于LEV组((214±48)μg/L,均P<0.01];各组间术后3、12、24 h TNF-α水平比较均无明显差异.与假手术组((135.43±40.08)μg/L]比较,模型组术后3 h血浆IL-6水平((267.65±72.87)μg/L]显著升高(P<0.01);GSH治疗组[(191.97±62.98)μg/L]较模型组显著降低,且明显低于LEV组[(268.75±74.67)μg/L,均P<0.05];各组间术后6、12、24 h IL-6水平均无明显差异.模型组大鼠肺组织超微结构发生显著变化,尤其是细胞内线粒体出现水肿甚至空泡变性;GSH组大鼠肺组织超微结构的改变轻微.结论 TNF-α和IL-6在脓毒症大鼠肺损伤发生机制中起重要作用,GSH有明显的治疗效果.
目的 探討還原型穀胱甘肽(GSH)對膿毒癥大鼠急性肺損傷保護作用的機製.方法 採用盲腸結扎穿孔術(CLP)複製SD大鼠膿毒癥模型.按隨機數字錶法將大鼠分為假手術組、模型組、GSH組、左氧氟沙星(LEV)組;分彆于術後3、6、12、24 h各取7隻大鼠心髒血檢測血漿腫瘤壞死因子-α(TNF-α)、白細胞介素-6(IL-6)水平,電鏡下觀察術後24 h大鼠肺組織超微結構的改變.結果 與假手術組C(132±9)μg/L]比較,模型組術後6 h血漿TNF-α水平((227±28)μg/L]顯著升高(zp<0.01);GSH治療組((144±28)μg/L]較模型組顯著降低,且明顯低于LEV組((214±48)μg/L,均P<0.01];各組間術後3、12、24 h TNF-α水平比較均無明顯差異.與假手術組((135.43±40.08)μg/L]比較,模型組術後3 h血漿IL-6水平((267.65±72.87)μg/L]顯著升高(P<0.01);GSH治療組[(191.97±62.98)μg/L]較模型組顯著降低,且明顯低于LEV組[(268.75±74.67)μg/L,均P<0.05];各組間術後6、12、24 h IL-6水平均無明顯差異.模型組大鼠肺組織超微結構髮生顯著變化,尤其是細胞內線粒體齣現水腫甚至空泡變性;GSH組大鼠肺組織超微結構的改變輕微.結論 TNF-α和IL-6在膿毒癥大鼠肺損傷髮生機製中起重要作用,GSH有明顯的治療效果.
목적 탐토환원형곡광감태(GSH)대농독증대서급성폐손상보호작용적궤제.방법 채용맹장결찰천공술(CLP)복제SD대서농독증모형.안수궤수자표법장대서분위가수술조、모형조、GSH조、좌양불사성(LEV)조;분별우술후3、6、12、24 h각취7지대서심장혈검측혈장종류배사인자-α(TNF-α)、백세포개소-6(IL-6)수평,전경하관찰술후24 h대서폐조직초미결구적개변.결과 여가수술조C(132±9)μg/L]비교,모형조술후6 h혈장TNF-α수평((227±28)μg/L]현저승고(zp<0.01);GSH치료조((144±28)μg/L]교모형조현저강저,차명현저우LEV조((214±48)μg/L,균P<0.01];각조간술후3、12、24 h TNF-α수평비교균무명현차이.여가수술조((135.43±40.08)μg/L]비교,모형조술후3 h혈장IL-6수평((267.65±72.87)μg/L]현저승고(P<0.01);GSH치료조[(191.97±62.98)μg/L]교모형조현저강저,차명현저우LEV조[(268.75±74.67)μg/L,균P<0.05];각조간술후6、12、24 h IL-6수평균무명현차이.모형조대서폐조직초미결구발생현저변화,우기시세포내선립체출현수종심지공포변성;GSH조대서폐조직초미결구적개변경미.결론 TNF-α화IL-6재농독증대서폐손상발생궤제중기중요작용,GSH유명현적치료효과.
Objective To investigate the protection mechanism of reduced glutathione (GSH) in acute lung injury in rats with sepsis.Methods Sepsis in Sprague-Dawley (SD) rats were reproduced by cecal ligation and puncture (CLP).They were randomly divided into four groups, sham-operated group, model group, GSH treatment group and levofloxacin (LEV) treatment group.Heart blood of 7 rats in all groups was collected at 3, 6, 12, 24 hours after operation.The plasma levels of tumor necrosis factor-α (TNF-α)and interleukin-6 (IL-6) were measured.The lung ultrastructure changes were observed with electron microscope at 24 hours in all groups.Results Compared with the sham-operated group, the plasma level of TNF-α increased more obviously at 6 hours of the model group [(227±28) μg/L vs.(132±9)μg/L, P<0.01].Compared with the model group, the plasma level of TNF-α in the GSH treatment group decreased obviously[(144± 28)μg/L], and it was obviously lower than that of LEV treatment group[(214±48) μg/L, both P<0.01].No obvious difference of plasma level of TNF-α was found at 3, 12, 24 hours among all the groups.Compared with the sham-operated group, the plasma level of IL-6 of the model group raised obviously at 3 hours [(267.65± 72.87)μg/L vs.(135.43±40.08)μg/L, P<0.01].In the GSH treatment group, the plasma level of IL-6 [(191.97±62.98) μg/L] was lower than that of the model group and the LEV treatment group [(268.75±74.67)μg/L, both P<0.05].The plasma level of IL-6 was not obviously different among all groups at 6, 12, 24 hours.In the model group, the injury of pulmonary ultrastructure was obvious, especially in the mitochondria of the pulmonary cells.In the GSH treatment group,the change in ultrastructure of the lung was slight.Conclusion TNF-α and IL-6 play significant role in the development of pulmonary ultrastructure injury in acute lung injury of septic rats.Treatment with GSH was effective in preventing such injury.
