CAJ | 학술논문

目的探讨P120catenin在胰腺癌中的表达及其基因T755G位点多态性与胰腺癌发病因素的关系.方法分别应用RT-PCR及Western Blot方法检测52例胰腺癌及癌旁正常胰腺组织中P120catenin的表达水平;应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对52例胰腺癌患者及60名正常对照的P120catenin基因T755G位点基因型分析.结果胰腺癌组P120catenin mRNA及蛋白表达与癌旁正常胰腺组相比均减少,差异有统计学意义(P=0.000,P=0.002);P120catenin mRNA异常表达更多见于低分化者(P=0.033)、淋巴转移者(P=0.004)、血管侵犯者(P=0.022)和高PTNM分期者(P=0.003).胰腺癌患者P120catenin基因T755G位点基因型频率及等位基因频率与正常组相比,差异有统计学意义(P=0.008,P=0.016).与TT基因型相比,GG基因型可增加胰腺癌发病风险(OR=2.765,95%CI为1.312～3.958).结论 P120catenin低表达与胰腺癌淋巴结转移、血管侵犯、pTNM分期密切相关,提示其可能在胰腺癌侵袭、转移过程中起重要作用.P120catenin基因T755G多态性与胰腺癌的发生风险相关,有利于胰腺癌的早期诊断与预防.
목적 탐토P120catenin재이선암중적표체급기기인T755G위점다태성여이선암발병인소적관계.방법 분별응용RT-PCR급Western Blot방법검측52례이선암급암방정상이선조직중P120catenin적표체수평;응용취합매련반응-한제성편단장도다태성(PCR-RFLP)기술대52례이선암환자급60명정상대조적P120catenin기인T755G위점기인형분석.결과 이선암조P120catenin mRNA급단백표체여암방정상이선조상비균감소,차이유통계학의의(P=0.000,P=0.002);P120catenin mRNA이상표체경다견우저분화자(P=0.033)、림파전이자(P=0.004)、혈관침범자(P=0.022)화고PTNM분기자(P=0.003).이선암환자P120catenin기인T755G위점기인형빈솔급등위기인빈솔여정상조상비,차이유통계학의의(P=0.008,P=0.016).여TT기인형상비,GG기인형가증가이선암발병풍험(OR=2.765,95%CI위1.312～3.958).결론 P120catenin저표체여이선암림파결전이、혈관침범、pTNM분기밀절상관,제시기가능재이선암침습、전이과정중기중요작용.P120catenin기인T755G다태성여이선암적발생풍험상관,유리우이선암적조기진단여예방.
Objectives To investigate the expression of P120catenin in pancreatic carcinoma and to explore the association between P120catenin gene polymorphism at T755G position and pancreatic carcinoma.Methods The expression of P120catenin in 52 cases of pancreatic carcinoma and normal pancreatic tissues on the mRNA and protein levels were evaluated by RT-PCR and Western Blot methods respectively.P120catenin gene polymorphism at 1755G position of in 52 patients and 60 healthy controls were examined by PCR-restriction fragment length polymorphism (PCR-RFLP) technique.Results The mRNA and protein expressions of P120catenin in pancreatic carcinoma tissues were significantly lower than normal pancreatic tissues (P=0.000,P=0.002).Reduced expression of P120catenin mRNA was significantly correlated with differentiated (P=0.033),lymph node metastasis (P=0.004),vascular invasion (P=0.022),and pTNM stage (P=0.003).Additionally,there were significant difference of P120catenin gene polymorphism genotypes and alleles at T755G position between patients and healthy controls(P=0.008,P=0.016).The GG genotype of P120catenin gene was associated with higher risk of incidence for pancreatic carcinoma compared with the TT genotype (OR=2.765,95% CI=1.312-3.958).Conclusions The reduced expressions of both P120 catenin mRNA and protein in pancreatic carcinoma suggest its association with pancreatic carcinoma development.Polymorphism of P120catenin gene at T755G situation might be a risk factor for pancreatic carcinoma,and it may be used to diagnosis and prevent pancreatic carcinoma early.