中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2010年
2期
132-137
,共6页
杨子峰%王玉涛%秦笙%罗翌%关文达%李际强%占扬清%张雪%周荣%莫自耀%陈敬贤%陈荣昌
楊子峰%王玉濤%秦笙%囉翌%關文達%李際彊%佔颺清%張雪%週榮%莫自耀%陳敬賢%陳榮昌
양자봉%왕옥도%진생%라익%관문체%리제강%점양청%장설%주영%막자요%진경현%진영창
正黏病毒科%病毒培养%呼吸道感染
正黏病毒科%病毒培養%呼吸道感染
정점병독과%병독배양%호흡도감염
Orthomyxoviridae%Vires cultivation%Respiratory tract infections
目的 应用高通量的小瓶离心法快速分离检测广州市成人流感样病例相关病毒,分析广州地区患有ILI的成人呼吸道病毒感染情况,评价其在临床实验室开展快速病毒培养的意义.方法 采集2009年1-6月广州市成人流感样病例患者标本652份,应用小瓶离心法(包括MDCK、HEp-2、LIE-MK_2和MRC-5 4种细胞),离心接种后,通过观察CPE、血凝(HA)、血吸附(HAD)及直接免疫荧光法(IF)等方法进行病毒分离鉴定.结果 652份成人流感样病例标本中共检出阳性161份,5种病毒病原体,病毒培养阳性率为24.69%,其中甲型流感病毒62株(62/161,38.51%),乙型流感病毒88株(88/161,54.65%),副流感病毒8株(8/161,4.96%),腺病毒2株(2/161,1.24%),呼吸道合胞病毒1株(1/161,0.62%).用小瓶离心培养法发出各种病毒培养报告的平均时间分别为:流感病毒2 d、腺病毒3 d、副流感病毒3 d.结论 (1)离心培养法具有快速、灵敏、高通量的特点,对临床实验室诊断病毒具有应用推广价值.(2)在分离的Iu病原体中,流感病毒占主导,乙型流感病毒为优势毒株,自2009年1月延续至6月,并呈逐步增多的态势,出现夏季高峰.
目的 應用高通量的小瓶離心法快速分離檢測廣州市成人流感樣病例相關病毒,分析廣州地區患有ILI的成人呼吸道病毒感染情況,評價其在臨床實驗室開展快速病毒培養的意義.方法 採集2009年1-6月廣州市成人流感樣病例患者標本652份,應用小瓶離心法(包括MDCK、HEp-2、LIE-MK_2和MRC-5 4種細胞),離心接種後,通過觀察CPE、血凝(HA)、血吸附(HAD)及直接免疫熒光法(IF)等方法進行病毒分離鑒定.結果 652份成人流感樣病例標本中共檢齣暘性161份,5種病毒病原體,病毒培養暘性率為24.69%,其中甲型流感病毒62株(62/161,38.51%),乙型流感病毒88株(88/161,54.65%),副流感病毒8株(8/161,4.96%),腺病毒2株(2/161,1.24%),呼吸道閤胞病毒1株(1/161,0.62%).用小瓶離心培養法髮齣各種病毒培養報告的平均時間分彆為:流感病毒2 d、腺病毒3 d、副流感病毒3 d.結論 (1)離心培養法具有快速、靈敏、高通量的特點,對臨床實驗室診斷病毒具有應用推廣價值.(2)在分離的Iu病原體中,流感病毒佔主導,乙型流感病毒為優勢毒株,自2009年1月延續至6月,併呈逐步增多的態勢,齣現夏季高峰.
목적 응용고통량적소병리심법쾌속분리검측엄주시성인류감양병례상관병독,분석엄주지구환유ILI적성인호흡도병독감염정황,평개기재림상실험실개전쾌속병독배양적의의.방법 채집2009년1-6월엄주시성인류감양병례환자표본652빈,응용소병리심법(포괄MDCK、HEp-2、LIE-MK_2화MRC-5 4충세포),리심접충후,통과관찰CPE、혈응(HA)、혈흡부(HAD)급직접면역형광법(IF)등방법진행병독분리감정.결과 652빈성인류감양병례표본중공검출양성161빈,5충병독병원체,병독배양양성솔위24.69%,기중갑형류감병독62주(62/161,38.51%),을형류감병독88주(88/161,54.65%),부류감병독8주(8/161,4.96%),선병독2주(2/161,1.24%),호흡도합포병독1주(1/161,0.62%).용소병리심배양법발출각충병독배양보고적평균시간분별위:류감병독2 d、선병독3 d、부류감병독3 d.결론 (1)리심배양법구유쾌속、령민、고통량적특점,대림상실험실진단병독구유응용추엄개치.(2)재분리적Iu병원체중,류감병독점주도,을형류감병독위우세독주,자2009년1월연속지6월,병정축보증다적태세,출현하계고봉.
Objective To evaluate the application of high-throughput shell vial assay in a clinical laboratory for detection of respiratory viruses from patients with ILI in Guangzhou between January and June, 2009. Methods Six hundred and fifty-two pharyngeal swab specimens were taken from ILI patents. Centrifugation-enhanced shell vials including 4 cell lines (MDCK, Hep-2, LLC-MK2 and MRC-5) were used for culture of respiratory viruses for 2-3 days. The cultures were identified by observation of cytopathic effect (CPE) , hemmaglution or hemmadsorption test as well as immunofluorescence staining. Results A total of 161 swab samples (24.69% ,161/652) were shown to have any one of the 5 common respiratory viruses including influenza A viruses ( 38. 51% , 62/161 ), influenza B virus ( 54. 65% , 88/161 ), parainfluenza viruses (4. 96% , 8/161 ) , adenovirus ( 1. 24% , 2/161 ), and respiratory syncytial virus (0. 62% ,1/161). The turnaround time was 2d for influenza viruses, 3d for adenovirus and parainfluenza viruses respectively. Conclusions (1) The shell vial method was effective, rapid and high throughout for the detection of respiratory viruses in clinical laboratories.(2)Influenza viruses were dominant in the swab samples from patients with ILI in Guangzhou between January and June with the highest appearance in the summer influenza B vires was the most common pathogen in patients with ILI in this study.
