中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2009年
24期
23-25
,共3页
阿托伐他汀%血管内皮细胞%增殖%FK506结舍蛋白12
阿託伐他汀%血管內皮細胞%增殖%FK506結捨蛋白12
아탁벌타정%혈관내피세포%증식%FK506결사단백12
Atorvastatin%Vascular endothelial cells%Proliferation%FKBP12
目的 探讨不同浓度阿托伐他汀对血管内皮细胞(VEC)增殖过程中FK506结合蛋白12(FKBP12)表达和细胞上清液ET-1的影响.方法 培养大鼠VEC并传代,观察其不同浓度阿托伐他汀作用下的不同时期FKBP12 mRNA水平的表达和细胞上清液ET-1的变化.结果 阿托伐他汀抑制血管内皮细胞增殖过程中FKBP12的表达,抑制血管内皮细胞合成和分泌ET-1;血管内皮细胞FKBP12表达和ET-1分泌基本一致,呈正相关,阿托伐他汀对它们的抑制作用且呈时间依赖性和剂量依赖性.结论 阿托伐他汀通过降低FKBP12表达抑制血管内皮细胞增殖并对ET-1合成和分泌起负性调节作用.
目的 探討不同濃度阿託伐他汀對血管內皮細胞(VEC)增殖過程中FK506結閤蛋白12(FKBP12)錶達和細胞上清液ET-1的影響.方法 培養大鼠VEC併傳代,觀察其不同濃度阿託伐他汀作用下的不同時期FKBP12 mRNA水平的錶達和細胞上清液ET-1的變化.結果 阿託伐他汀抑製血管內皮細胞增殖過程中FKBP12的錶達,抑製血管內皮細胞閤成和分泌ET-1;血管內皮細胞FKBP12錶達和ET-1分泌基本一緻,呈正相關,阿託伐他汀對它們的抑製作用且呈時間依賴性和劑量依賴性.結論 阿託伐他汀通過降低FKBP12錶達抑製血管內皮細胞增殖併對ET-1閤成和分泌起負性調節作用.
목적 탐토불동농도아탁벌타정대혈관내피세포(VEC)증식과정중FK506결합단백12(FKBP12)표체화세포상청액ET-1적영향.방법 배양대서VEC병전대,관찰기불동농도아탁벌타정작용하적불동시기FKBP12 mRNA수평적표체화세포상청액ET-1적변화.결과 아탁벌타정억제혈관내피세포증식과정중FKBP12적표체,억제혈관내피세포합성화분비ET-1;혈관내피세포FKBP12표체화ET-1분비기본일치,정정상관,아탁벌타정대타문적억제작용차정시간의뢰성화제량의뢰성.결론 아탁벌타정통과강저FKBP12표체억제혈관내피세포증식병대ET-1합성화분비기부성조절작용.
Objective To evaluate the effect of atorvastatin on mRNA expression of FK506 binding protein 12 (FKBP12) in vascular endothelial cell(VEC) proliferation and the concentration of ET-1 in supernatant fluid of endothelial cell.Methods Rat vascular endothelial cells were cultured with enzyme digesting methods,and were identified by measuring their growth rate with invertmicroscope,by using immuocytochemical methods with factor Ⅷ antibody.Then the cells were treated with atorvastatin in different concentration.The proliferation of rat vascular endothelial cell was observed under an invert microscope.The expression of FKBP12 mRNA and the concentration of ET-1 in supernatant fluid of endothelial cell were detected in different culture periods.Results Atorvastatin markedly restrained the expressions of FKBP12 mRNA in VEC and the concentration of ET-1 in supernatant fluid of endothelial cell.A significantly positive correlation of them was indentified.The effect appeared dose dependence and time dependence.Conclusions Atorvastatin dose-dependently and time-dependently restrained the proliferation of rat VEC.As a result,the expression of FKBP12 mRNA and the concentration of ET-1 in supernatant fluid of endothelial cell are decreased too.
