CAJ | 학술논문

实验所用巴西盾柱木种子由圣保罗州Anhembi公司提供,种子经由98%的浓硫酸预处理15 min, 以打破物理休眠.分别在0.2%克菌丹溶液10°C和27°C下,-1.0 MPa聚乙二醇6000 10°C和27°C下,以及, 0.5 mol,0.75 mol,1.0 mol 硝酸钾溶液进行引物处理. 包括对照组种子,共8个实验处理,每一个处理5个重复,每一个处理实验用100粒种子,置于滤纸浸透引物聚乙二醇6000溶液的15厘米培养皿中,置于冰箱保持27°C恒温.实验引物渗透势为0.0, -0.2, -0.4, -0.6, -0.8, -1.0-MPa, -1.4 MPa. 实验同时将种子置于浸有引物聚乙二醇的卷纸中,观察水胁迫下的萌发情况.实验结果证明,随着聚乙二醇6000浓度的升高,巴西盾柱木种子萌发率降低,对照组萌发率比实验引物处理组高.聚乙二醇达到PEG -1.4 MPa,种子不能萌发.图2表3参10.
실험소용파서순주목충자유골보라주Anhembi공사제공,충자경유98%적농류산예처리15 min, 이타파물리휴면.분별재0.2%극균단용액10°C화27°C하,-1.0 MPa취을이순6000 10°C화27°C하,이급, 0.5 mol,0.75 mol,1.0 mol 초산갑용액진행인물처리. 포괄대조조충자,공8개실험처리,매일개처리5개중복,매일개처리실험용100립충자,치우려지침투인물취을이순6000용액적15전미배양명중,치우빙상보지27°C항온.실험인물삼투세위0.0, -0.2, -0.4, -0.6, -0.8, -1.0-MPa, -1.4 MPa. 실험동시장충자치우침유인물취을이순적권지중,관찰수협박하적맹발정황.실험결과증명,수착취을이순6000농도적승고,파서순주목충자맹발솔강저,대조조맹발솔비실험인물처리조고.취을이순체도PEG -1.4 MPa,충자불능맹발.도2표3삼10.
Peltophorum dubium seeds provided by Anhembi, SP were scarified in 98% H2SO4 for 15 min to overcome mechanical dormancy. Seeds were primed in solutions of 0.2% Captan at 10 °C and 27°C, PEG 6000 -1.0 MPa at 10°C and 27°C, 0.5 mol KNO3, 0.75 Mol KNO3, 1.0 Mol KNO3. Eight treatments including the primed seeds and nonprimed seeds, five replicates with 100 seeds for each treatment, were set to 15-cm-Petri dish with double filter paper moistened with testing solution PEG in refrigerator at 27°C. For the experiments of all the groups, osmotic potential were 0.0, -0.2, -0.4, -0.6, -0.8, -1.0, -1.2, and -1.4 MPa. P. dubium seeds were also set to water stress experiment in rolled paper with PEG solutions from 0.0 to -1.0 M Pa. Germination percentage decreased with the increase of PEG concentration. Control group had a better germination percentage than other groups. Germination hardly occurred in PEG -1.4 MPa.