CAJ | 학술논문

以采自浙江省丽水山区的4个红菇科菌株作为研究材料,进行rDNA ITS(Internal Transcribed Spacer)区段克隆测序和序列特征比较分析,并对ITS序列进行核酸序列数据库GenBank同源性检索比对,将从GenBank检索获得的15个最相似物种的ITS序列连同4个红菇科菌株的ITS序列一起用于系统发育分析.序列比较结果表明,4个红菇科菌株的rDNA ITS序列长度为673 bp～766 bp,GC含量为47.4%～49.48%,其中2个红菇属(Russula)菌株R32和R57间的ITS序列长度和GC含量差异极小,而与血红乳菇(Lactarius sanguifluus)菌株L37间的ITS序列长度和GC含量差异较大.系统发育分析表明,R57为花盖红菇(R. cyanoxantha)的一个菌株,红菇属的赭盖红菇(R. mustelina)、绿菇(R. virescens)和青灰红菇(R. parazurea)三者间的序列差异较小,亲缘关系较近;乳菇属(Lactarius)的血红乳菇同鲑色乳菇(L. salmonicolor)种间的序列差异较小,亲缘关系较近.
이채자절강성려수산구적4개홍고과균주작위연구재료,진행rDNA ITS(Internal Transcribed Spacer)구단극륭측서화서렬특정비교분석,병대ITS서렬진행핵산서렬수거고GenBank동원성검색비대,장종GenBank검색획득적15개최상사물충적ITS서렬련동4개홍고과균주적ITS서렬일기용우계통발육분석.서렬비교결과표명,4개홍고과균주적rDNA ITS서렬장도위673 bp～766 bp,GC함량위47.4%～49.48%,기중2개홍고속(Russula)균주R32화R57간적ITS서렬장도화GC함량차이겁소,이여혈홍유고(Lactarius sanguifluus)균주L37간적ITS서렬장도화GC함량차이교대.계통발육분석표명,R57위화개홍고(R. cyanoxantha)적일개균주,홍고속적자개홍고(R. mustelina)、록고(R. virescens)화청회홍고(R. parazurea)삼자간적서렬차이교소,친연관계교근;유고속(Lactarius)적혈홍유고동해색유고(L. salmonicolor)충간적서렬차이교소,친연관계교근.
Internal transcribed spacer (ITS) sequences of four edible and medicinal fungi belonging to the Russulaceae, collected from the Lishui mountainous area of Zhejiang Province, were cloned and sequenced. Comparisons with sequence data in GenBank were undertaken using BLAST, and the four ITS sequences, together with 15 reference sequences obtained from GenBank, were used to construct a phylogenetic tree. rDNA ITS sequences of the four strains ranged from 673 to 766 bp in length and between 47.4% and 49.48% in GC content. Phylogenetic data indicated strain R57 to be Russula cyanoxantha. Differences in the size and GC content of ITS sequences from strains R32 and R57 (R. cyanoxantha) and strain L37 (Lactarius sanguifluus) were significant, whereas the corresponding differences between strains R32 and R57 were relatively small. A close kinship, with only small detectable differences in their respective ITS sequences, existed between R. mustelina, R. virescens and R. parazurea. A similar situation applied to strains of L. sanguifluus and L. salmonicolor.